Nonmuscle myosin II molecules carry out a wide variety of functions within cells. There are three nonmuscle myosin II genes. We have studied the strain dependence of the ADP release mechanism in nonmuscle myosin IIA and IIB using a solution kinetic approach. The results show that both positive strains accelerate the rate of ADP release whereas negative strain markedly lowers the rate. These results suggest that these myosins, and particularly nonmuscle myosin IIB, are ideally suited to act as tension bearers in cells.

National Institute of Health (NIH)
National Heart, Lung, and Blood Institute (NHLBI)
Intramural Research (Z01)
Project #
Application #
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
U.S. National Heart Lung and Blood Inst
United States
Zip Code
Young, Gavin; Hundt, Nikolas; Cole, Daniel et al. (2018) Quantitative mass imaging of single biological macromolecules. Science 360:423-427
Bond, Lisa M; Sellers, James R; McKerracher, Lisa (2015) Rho kinase as a target for cerebral vascular disorders. Future Med Chem 7:1039-53
Heissler, Sarah M; Sellers, James R (2015) Four things to know about myosin light chains as reporters for non-muscle myosin-2 dynamics in live cells. Cytoskeleton (Hoboken) 72:65-70
Billington, Neil; Revill, Derek J; Burgess, Stan A et al. (2014) Flexibility within the heads of muscle myosin-2 molecules. J Mol Biol 426:894-907
Kim, Kye-Young; Kawamoto, Sachiyo; Bao, Jianjun et al. (2008) The B2 alternatively spliced isoform of nonmuscle myosin II-B lacks actin-activated MgATPase activity and in vitro motility. Biochem Biophys Res Commun 369:124-34
Sellers, James R; Knight, Peter J (2007) Folding and regulation in myosins II and V. J Muscle Res Cell Motil 28:363-70
Iwamoto, Hiroyuki; Oiwa, Kazuhiro; Kovacs, Mihaly et al. (2007) Diversity of structural behavior in vertebrate conventional myosins complexed with actin. J Mol Biol 369:249-64
Kovacs, Mihaly; Thirumurugan, Kavitha; Knight, Peter J et al. (2007) Load-dependent mechanism of nonmuscle myosin 2. Proc Natl Acad Sci U S A 104:9994-9
Kim, Kye-Young; Kovacs, Mihaly; Kawamoto, Sachiyo et al. (2005) Disease-associated mutations and alternative splicing alter the enzymatic and motile activity of nonmuscle myosins II-B and II-C. J Biol Chem 280:22769-75
Kovacs, Mihaly; Wang, Fei; Hu, Aihua et al. (2003) Functional divergence of human cytoplasmic myosin II: kinetic characterization of the non-muscle IIA isoform. J Biol Chem 278:38132-40

Showing the most recent 10 out of 14 publications