Progress during FY18: 1) Completed and submitted for publication an analysis of NF-B target genes in PMA plus calcium ionophore-activated BJAB, a human B lymphoma cell line. We identified approximately 300 target genes that were up-regulated by NF-B. These genes had different kinetic patterns of activation, and we provided plausible mechanisms by which these patterns were generated. We also identified approximately 500 genes whose induction required NF-B, but which did not bind the transcription factor. We termed these indirect targets of NF-B that were activated by NF-B-induced transcription factors. A manuscript describing these observations is in press in PLOS Biology. 2) We used the pipeline established by the BJAB studies to query primary murine B lymphocytes for NF-B target genes regulated by signals from the B cell antigen receptor (BCR) or Toll-like receptor 4 (TLR4). This analysis will likely provide insights into mechanisms that establish stimulus-specificity of transcription responses, because both BCR and TLR4 activate NF-B but induce distinct gene regulatory outcomes. 3) We used Rel-deficient murine B lymphocytes in combined transcriptional and ChIP-Seq studies to understand the molecular basis for subunit-specific NF-B responses (in this case a comparison of Rel- and RelA-dependent gene expression). 4) Generation of conditional Gtf2ifL mice: - Gtf2ifL mice were bred to BL6 mice for 10 generations and named Gtf2ifL(G10B6). After the 10th generation they were breed to each other to have the fl/fl as HO (homozygous) (done before October 01, 2015). - Gtf2ifL(G10B6) were bred to the B-cell specific CD19cre, the pups were named Gtf2ifLxCD19-Cre. All litters of this strain were either Gtf2ifL Ht/CD19-Cre+ or Ht/CD19-Cre-. (Htheterozygous) - Gtf2ifLxCD19-Cre Ht/Cre+ were bred with Gtf2ifLxCD19-Cre Ht/Cre-, two breeding pairs of these mice gave 3 litters. - 6 pairs of Gtf2ifL Ht/CD19-Cre+ were bred to Gtf2ifL(G10B6) HO, one litter of 4 pups was weaned. Several pups exhibited correct genotype and were subsequently used for the following experiments. - ATAC- and RNA-Seq experiments were carried out, data analysis is in progress. Preliminary results show an interesting effect of ATF3 up-regulation in marginal zone B cells from Gtf2i-/-/ mice.
| Lee-Chang, Catalina; Bodogai, Monica; Moritoh, Kanako et al. (2016) Aging Converts Innate B1a Cells into Potent CD8+ T Cell Inducers. J Immunol 196:3385-97 |
| Kaileh, Mary; Vazquez, Estefania; MacFarlane 4th, Alexander W et al. (2016) mTOR-Dependent and Independent Survival Signaling by PI3K in B Lymphocytes. PLoS One 11:e0146955 |
| Fowler, Trent; Garruss, Alexander S; Ghosh, Amalendu et al. (2015) Divergence of transcriptional landscape occurs early in B cell activation. Epigenetics Chromatin 8:20 |
| Maul, Robert W; Cao, Zheng; Venkataraman, Lakshmi et al. (2014) Spt5 accumulation at variable genes distinguishes somatic hypermutation in germinal center B cells from ex vivo-activated cells. J Exp Med 211:2297-306 |
| Kaileh, Mary; Sen, Ranjan (2012) NF-ýýB function in B lymphocytes. Immunol Rev 246:254-71 |
| Olkhanud, Purevdorj B; Damdinsuren, Bazarragchaa; Bodogai, Monica et al. (2011) Tumor-evoked regulatory B cells promote breast cancer metastasis by converting resting CD4? T cells to T-regulatory cells. Cancer Res 71:3505-15 |
| Fowler, Trent; Sen, Ranjan; Roy, Ananda L (2011) Regulation of primary response genes. Mol Cell 44:348-60 |
| Sen, Ranjan (2011) The origins of NF-?B. Nat Immunol 12:686-8 |
| Damdinsuren, Bazarragchaa; Zhang, Yongqing; Khalil, Ashraf et al. (2010) Single round of antigen receptor signaling programs naive B cells to receive T cell help. Immunity 32:355-66 |
| Sen, Ranjan; Smale, Stephen T (2010) Selectivity of the NF-{kappa}B response. Cold Spring Harb Perspect Biol 2:a000257 |
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