Abstract

In FY 2014, we continued investigation of the staphylococcal peptide cytolysins, phenol-soluble modulins (PSMs). Current efforts focus on structure-function relationship studies of PSMs, antimicrobial activities of PSMs, and the PSM exporter, with the long-term goals to produce anti-PSM therapeutics for the treatment of staphylococcal infections. We have identified the PSM exporter and are currently setting up translational follow-up projects to find drugs interfering with PSM export. Furthermore, we analyzed the basis of virulence in a global community-assocoiated MRSA strain (ST72 from Korea), which does not harbor the genes encoding the Panton-Valentine leukocidin. Finally, we found that the production of a specific allelic variant of the most cytolytic and pro-inflammatory PSM, PSM alpha , is linked to an MRSA lineage that is a frequent cause of sepsis.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Investigator-Initiated Intramural Research Projects (ZIA)
Project #
1ZIAAI000904-13
Application #
8946357
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
13
Fiscal Year
2014
Total Cost
Indirect Cost

  Institution

Name
Niaid Extramural Activities
Department
Type
DUNS #
City
State
Country
Zip Code

  Publications

Zheng, Yue; He, Lei; Asiamah, Titus K et al. (2018) Colonization of medical devices by staphylococci. Environ Microbiol 20:3141-3153
He, Lei; Zheng, Hong-Xiang; Wang, Yanan et al. (2018) Detection and analysis of methicillin-resistant human-adapted sequence type 398 allows insight into community-associated methicillin-resistant Staphylococcus aureus evolution. Genome Med 10:5
Fisher, Emilie L; Otto, Michael; Cheung, Gordon Y C (2018) Basis of Virulence in Enterotoxin-Mediated Staphylococcal Food Poisoning. Front Microbiol 9:436
Jones, Dennis; Meijer, Eelco F J; Blatter, Cedric et al. (2018) Methicillin-resistant Staphylococcus aureus causes sustained collecting lymphatic vessel dysfunction. Sci Transl Med 10:
Schlatterer, Katja; Beck, Christian; Hanzelmann, Dennis et al. (2018) The Mechanism behind Bacterial Lipoprotein Release: Phenol-Soluble Modulins Mediate Toll-Like Receptor 2 Activation via Extracellular Vesicle Release from Staphylococcus aureus. MBio 9:
Nakatsuji, Teruaki; Chen, Tiffany H; Butcher, Anna M et al. (2018) A commensal strain of Staphylococcus epidermidis protects against skin neoplasia. Sci Adv 4:eaao4502
Zheng, Yue; Joo, Hwang-Soo; Nair, Vinod et al. (2018) Do amyloid structures formed by Staphylococcus aureus phenol-soluble modulins have a biological function? Int J Med Microbiol 308:675-682
Cheung, Gordon Y C; Fisher, Emilie L; McCausland, Joshua W et al. (2018) Antimicrobial Peptide Resistance Mechanism Contributes to Staphylococcus aureus Infection. J Infect Dis 217:1153-1159
Blake, Kimbria J; Baral, Pankaj; Voisin, Tiphaine et al. (2018) Staphylococcus aureus produces pain through pore-forming toxins and neuronal TRPV1 that is silenced by QX-314. Nat Commun 9:37
Piewngam, Pipat; Zheng, Yue; Nguyen, Thuan H et al. (2018) Pathogen elimination by probiotic Bacillus via signalling interference. Nature 562:532-537

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