Abstract

Respiratory syncytial virus (RSV) is the leading cause of lower respiratory tract infections in infants and small children. RSV infection is characterized by significant immunopathology, which is mediated by CD8+ cytotoxic T lymphocytes (CTL). Past studies have suggested that the multiple mechanisms employed by CTLs to eliminate cells may have differential effects on viral clearance and RSV-associated illness. This study seeks to define the contributions of the different CTL effector mechanisms and CD25+ T regulatory cells to the clearance of primary RSV infection, as well as their relative impact on the virus-associated immunopathology.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Investigator-Initiated Intramural Research Projects (ZIA)
Project #
1ZIAAI005027-09
Application #
8148428
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
9
Fiscal Year
2010
Total Cost
$940,148
Indirect Cost

  Institution

Name
National Institute of Allergy and Infectious Diseases
Department
Type
DUNS #
City
State
Country
Zip Code

  Publications

Ruckwardt, T J; Morabito, K M; Bar-Haim, E et al. (2018) Neonatal mice possess two phenotypically and functionally distinct lung-migratory CD103+ dendritic cell populations following respiratory infection. Mucosal Immunol 11:186-198
Morabito, Kaitlyn M; Ruckwardt, Tracy J; Bar-Haim, Erez et al. (2018) Memory Inflation Drives Tissue-Resident Memory CD8+ T Cell Maintenance in the Lung After Intranasal Vaccination With Murine Cytomegalovirus. Front Immunol 9:1861
Malloy, Allison M W; Ruckwardt, Tracy J; Morabito, Kaitlyn M et al. (2017) Pulmonary Dendritic Cell Subsets Shape the Respiratory Syncytial Virus-Specific CD8+ T Cell Immunodominance Hierarchy in Neonates. J Immunol 198:394-403
Morabito, K M; Ruckwardt, T R; Redwood, A J et al. (2017) Intranasal administration of RSV antigen-expressing MCMV elicits robust tissue-resident effector and effector memory CD8+ T cells in the lung. Mucosal Immunol 10:545-554
Ruckwardt, Tracy J; Morabito, Kaitlyn M; Graham, Barney S (2016) Determinants of early life immune responses to RSV infection. Curr Opin Virol 16:151-157
Morabito, Kaitlyn M; Erez, Noam; Graham, Barney S et al. (2016) Phenotype and Hierarchy of Two Transgenic T Cell Lines Targeting the Respiratory Syncytial Virus KdM282-90 Epitope Is Transfer Dose-Dependent. PLoS One 11:e0146781
Pawlak, Joanna B; Gential, Geoffroy P P; Ruckwardt, Tracy J et al. (2015) Bioorthogonal deprotection on the dendritic cell surface for chemical control of antigen cross-presentation. Angew Chem Int Ed Engl 54:5628-31
Ruckwardt, Tracy J; Malloy, Allison M W; Morabito, Kaitlyn M et al. (2014) Quantitative and qualitative deficits in neonatal lung-migratory dendritic cells impact the generation of the CD8+ T cell response. PLoS Pathog 10:e1003934
Bar-Haim, Erez; Erez, Noam; Malloy, Allison M W et al. (2014) CD8+ TCR transgenic strains expressing public versus private TCR targeting the respiratory syncytial virus K(d)M2(82-90) epitope demonstrate similar functional profiles. PLoS One 9:e99249
Billam, Padma; Bonaparte, Kathryn L; Liu, Jie et al. (2011) T Cell receptor clonotype influences epitope hierarchy in the CD8+ T cell response to respiratory syncytial virus infection. J Biol Chem 286:4829-41

Showing the most recent 10 out of 15 publications