Inhibitors directed against three distinct points in the HIV-1 life cycle are being prepared. These inhibitors are intended to serve as potential new therapeutics and as pharmacological probes to investigate biochemical mechanisms of viral replication. The three areas of investigation are: (1) HIV-1 integrase (IN), where inhibitors may disrupt incorporation of viral cDNA into the host genome;(2) Binding of HIV p6Gag protein to human Tsg101 protein, where inhibitors may disrupt viral assembly and budding and (3) Binding of human cytidine deaminase APOBEC3G (A3G) to the HIV-1 protein Vif, where inhibitors may result in hyper-mutation of proviral DNA. (1) HIV-1 IN inhibitors: Although a large number of inhibitors have been reported in the literature, diketoacid (DKA)-derived agents have recently shown particular promise, with members of this genre showing significant promise in anti-AIDS clinical trials. These agents are thought to function by chelating Mg2+ ions at the IN catalytic site, where they selectively inhibit strand transfer (ST) reactions over 3-processing (3-P) reactions. We had previously reported the bis-salicylhydrazides class of IN inhibitors that we also hypothesized function by metal chelation. However, members of this series exhibit potent inhibition only when Mn2+ is used as cofactor and not when the physiologically-relevant Mg2+ is used. Our recent findings have shown that bis-aroylhydrazides could acquire inhibitory potency in the presence of Mg2+ through the inclusion of dihdroxybenzoyl substituents. Good selectivity for IN-catalyzed ST versus 3-P reactions could also be achieved using a 2,3-dihydro-6,7-dihydroxy-1H-isoindol-1-one ring system as a conformationally-constrained 2,3-dihydroxybenzoyl equivalent. Adding a second oxo-group to the isoindole-1-one ring system gave the phthalimide-based isoindole-1,3(2H)-diones, which generally exhibited enhanced of 3-P and ST inhibitory potencies. Antiviral effects in cultured cells using HIV-1 based vectors showed that the certain bicyclic conformationally constrained analogues exhibited sub-micromolar antiviral potencies against HIV-1 infected cells. This work indicates that the 4,5-dihydroxyphthalimide nucleus offers a structurally simple starting point for the further development of IN inhibitors. Synthesis has been performed of a large number of analogues that broadly fall into two structural classes: (1) Halogenated N-aryl compounds and (2) chiral N-benzamide compounds. To reduce unwanted cytotoxicity, recent work has focused on modifying the 4,5-dihydroxyphthalimide nucleus by introducing a nitrogen atom into the aryl ring. (2) Tsg101-binding inhibitors: Binding of the HIV p6Gag protein to human Tsg101 protein has been shown to be necessary for viral budding and to involve a critical 9-mer P-E-P-T-A-P-P-E-E sequence of the p6 protein. We are preparing peptide and peptide mimetic variants of this 9-mer sequence as Tsg101-binding antagonists that may lead to a new class of viral budding inhibitors. In a collaboration with Dr. Eric Freed (CCR, NCI, NIH) of our approaches has been to replace the Pro4 residue with N-substitued glycine (NSG) residues (termed peptoids). However, this is synthetically problematic. Therefore, we resorted to a new family of peptoid variants that incorporate oximes and hydrazone amides as NSG surrogates. These can be prepared readily in library fashion by reacting a series of aldehydes with a single HPLC-purified precursor following cleavage from the solid-phase resin. We have extended these studies by replacing several key residues in the parent p6-derived 9-mer sequence with other non-natural amino acid analogues. A unifying principal guiding our approach has been the incorporation of amino-oxy functionality into the residues that can be functionalized in a single final step to provide a library of oxime derivatives. This approach has resulted in the identification of several low micromolar affinity Tsg101 binding antagonists. Fluorescent labeling has been introduced into one of the high affinity-binding antagonists to produce a reagent that will be used for identification of Tsg101-binding antagonists by high throughput screening of small compound libraries. In parallel work the chemical biology of Tsg101 interactions with peptide ligands has been explored using conformationally-constrained peptide macrocycles. These were formed by ring-closing metathesis reactions on peptide-peptoid precursors. These macrocycles exhibit higher Tsg101-binding affinity and better cellular bioavailability than the parent open-chain peptides. (3) Inhibitors of proteosomal targeting of human cytidine deaminase APOBEC3G (A3G) binding by the HIV-1 protein Vif: After infection of a target cell and during reverse transcription of HIV-1 RNA into DNA, the human A3G enzyme deaminates C residues in the minus strand, converting them to U residues. This results in encoding of A residues in the plus strand DNA in place of the original G residues, with the net effect being hypermutation of proviral DNA. The HIV-1 23 kDa cytoplasmic protein Vif overcomes the antiviral effects of A3G by binding to it and targeting it for proteasomal degradation. The laboratory of Dr. Vinay Pathak (CCR, NCI, NIH) has shown that the Vif protein sequence S(144)LQYLA(149) is critical for targeting A3G to the proteosome. Our initial work has been to prepare fluoresceine isothiocyanate (FITC)-derived peptide conjugates for use in the laboratory of Dr. Robert Fisher (SAIC, Inc., NCI-Frederick) to develop Vif fluorescence anisotropy binding assays. In collaboration with Dr. Pathaks laboratory, we have conducted structure-activity studies on the SLQYLA sequence in an effort to develop higher affinity, more cell-permeable analogues that can effectively block proteosomal targeting of A3G by Vif. Among the techniques used in this work is the use of oxime peptide libraries as described above for Tsg101-binding inhibitors.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Investigator-Initiated Intramural Research Projects (ZIA)
Project #
1ZIABC007363-15
Application #
7965099
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
15
Fiscal Year
2009
Total Cost
$408,065
Indirect Cost
Name
National Cancer Institute Division of Basic Sciences
Department
Type
DUNS #
City
State
Country
Zip Code
Zhao, Xue Zhi; Smith, Steven J; Maskell, Daniel P et al. (2017) Structure-Guided Optimization of HIV Integrase Strand Transfer Inhibitors. J Med Chem 60:7315-7332
Métifiot, Mathieu; Johnson, Barry C; Kiselev, Evgeny et al. (2016) Selectivity for strand-transfer over 3'-processing and susceptibility to clinical resistance of HIV-1 integrase inhibitors are driven by key enzyme-DNA interactions in the active site. Nucleic Acids Res 44:6896-906
Zhao, Xue Zhi; Smith, Steven J; Maskell, Daniel P et al. (2016) HIV-1 Integrase Strand Transfer Inhibitors with Reduced Susceptibility to Drug Resistant Mutant Integrases. ACS Chem Biol 11:1074-81
Yan, Zhipeng; Bryant, Kevin F; Gregory, Sean M et al. (2014) HIV integrase inhibitors block replication of alpha-, beta-, and gammaherpesviruses. MBio 5:e01318-14
Métifiot, Mathieu; Maddali, Kasthuraiah; Johnson, Barry C et al. (2013) Activities, crystal structures, and molecular dynamics of dihydro-1H-isoindole derivatives, inhibitors of HIV-1 integrase. ACS Chem Biol 8:209-17
Zhao, Xue Zhi; Maddali, Kasthuraiah; Smith, Steven J et al. (2012) 6,7-Dihydroxy-1-oxoisoindoline-4-sulfonamide-containing HIV-1 integrase inhibitors. Bioorg Med Chem Lett 22:7309-13
Zhao, Xue Zhi; Maddali, Kasthuraiah; Metifiot, Mathieu et al. (2012) Bicyclic hydroxy-1H-pyrrolopyridine-trione containing HIV-1 integrase inhibitors. Chem Biol Drug Des 79:157-65
Zhao, Xue Zhi; Maddali, Kasthuraiah; Metifiot, Mathieu et al. (2011) Development of tricyclic hydroxy-1H-pyrrolopyridine-trione containing HIV-1 integrase inhibitors. Bioorg Med Chem Lett 21:2986-90
Kim, Sung-Eun; Liu, Fa; Im, Young Jun et al. (2011) Elucidation of New Binding Interactions with the Tumor Susceptibility Gene 101 (Tsg101) Protein Using Modified HIV-1 Gag-p6 Derived Peptide Ligands. ACS Med Chem Lett 2:337-341
Métifiot, Mathieu; Johnson, Barry; Smith, Steven et al. (2011) MK-0536 inhibits HIV-1 integrases resistant to raltegravir. Antimicrob Agents Chemother 55:5127-33

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