Abstract

a) Profile tumors in terms of tumor vascular permeability and oxygenation using in vivo hyperpolarization of water at low fields. Hyperpolarization is usually carried out with the paramagnetic molecule TAM. From the image intensity profiles we obtained tumor oxygen and tumor vascular permeability simultaneously and correlated with immunohistochemical characterization of tumors. We found that tumor hypoxia was associated with increased tumor vascular permeability which in turn was negatively correlated with vascular pericyte coverage. b) Monitor the in vivo breakdown products of hyperpolarized (ex vivo) 13C-labeled pyruvic acid in tumors: In this study, we injected 13C labeled pyruvate and monitored the conversion to lactate. The pyruvate/lactate ratio was quantified in the tumor and correlated with oxygen maps from the tumor obtained from EPRI.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Investigator-Initiated Intramural Research Projects (ZIA)
Project #
1ZIABC010477-07
Application #
7965338
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
7
Fiscal Year
2009
Total Cost
$379,028
Indirect Cost

  Institution

Name
National Cancer Institute Division of Basic Sciences
Department
Type
DUNS #
City
State
Country
Zip Code

  Publications

Read, Graham H; Miura, Natsuko; Carter, Jenna L et al. (2018) Three-dimensional alginate hydrogels for radiobiological and metabolic studies of cancer cells. Colloids Surf B Biointerfaces 171:197-204
Matsumoto, Ken-Ichiro; Mitchell, James B; Krishna, Murali C (2018) Comparative studies with EPR and MRI on the in vivo tissue redox status estimation using redox-sensitive nitroxyl probes: influence of the choice of the region of interest. Free Radic Res 52:248-255
Kishimoto, Shun; Bernardo, Marcelino; Saito, Keita et al. (2015) Evaluation of oxygen dependence on in vitro and in vivo cytotoxicity of photoimmunotherapy using IR-700-antibody conjugates. Free Radic Biol Med 85:24-32
Enomoto, Ayano; Hirata, Hiroshi; Matsumoto, Shingo et al. (2014) Four-channel surface coil array for 300-MHz pulsed EPR imaging: proof-of-concept experiments. Magn Reson Med 71:853-8
Jang, Hyungseok; Subramanian, Sankaran; Devasahayam, Nallathamby et al. (2013) Single acquisition quantitative single-point electron paramagnetic resonance imaging. Magn Reson Med 70:1173-81
Krishna, Murali C; Matsumoto, Shingo; Saito, Keita et al. (2013) Magnetic resonance imaging of tumor oxygenation and metabolic profile. Acta Oncol 52:1248-56
Matsumoto, Shingo; Saito, Keita; Yasui, Hironobu et al. (2013) EPR oxygen imaging and hyperpolarized 13C MRI of pyruvate metabolism as noninvasive biomarkers of tumor treatment response to a glycolysis inhibitor 3-bromopyruvate. Magn Reson Med 69:1443-50
Matsumoto, Shingo; Batra, Sonny; Saito, Keita et al. (2011) Antiangiogenic agent sunitinib transiently increases tumor oxygenation and suppresses cycling hypoxia. Cancer Res 71:6350-9
Day, Sam E; Kettunen, Mikko I; Cherukuri, Murali Krishna et al. (2011) Detecting response of rat C6 glioma tumors to radiotherapy using hyperpolarized [1- 13C]pyruvate and 13C magnetic resonance spectroscopic imaging. Magn Reson Med 65:557-63