Abstract

We have transitioned from evaluating the skin bacterial microbiome of multiple skin sites in healthy adults to investigating the relevance of the skin microbiome fluctuations to skin diseases. In fiscal year 2011, we enrolled 21 new subjects to study the microbiome of patients with atopic dermatitis (eczema) and primary immunodeficiencies with dermatitis (hyper-IgE syndrome and Wiskott-Aldrich syndrome), in addition to follow-up sampling visits from previously enrolled subjects. Evaluations include complete skin examination, scoring using the SCORing Atopic Dermatitis (SCORAD) system for moderate to severe atopic dermatitis disease, bloodwork, and digital photography. The sequencing for this project is funded by the National Institutes of Health Roadmap Project, The Human Microbiome Project. We have been analyzing the microbiome data from patients with atopic dermatitis, hyper-IgE syndrome and Wiskott-Aldrich syndrome in the last fiscal year. We have also begun to study the fungal microbiome in healthy adults.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Investigator-Initiated Intramural Research Projects (ZIA)
Project #
1ZIABC010938-04
Application #
8349237
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
4
Fiscal Year
2011
Total Cost
$473,066
Indirect Cost

  Institution

Name
National Cancer Institute Division of Basic Sciences
Department
Type
DUNS #
City
State
Country
Zip Code

  Publications

Shah, Nirali N; Freeman, Alexandra F; Su, Helen et al. (2017) Haploidentical Related Donor Hematopoietic Stem Cell Transplantation for Dedicator-of-Cytokinesis 8 Deficiency Using Post-Transplantation Cyclophosphamide. Biol Blood Marrow Transplant 23:980-990
Kennedy, Elizabeth A; Connolly, Jennifer; Hourihane, Jonathan O'B et al. (2017) Skin microbiome before development of atopic dermatitis: Early colonization with commensal staphylococci at 2 months is associated with a lower risk of atopic dermatitis at 1 year. J Allergy Clin Immunol 139:166-172
Kong, Heidi H; Andersson, Björn; Clavel, Thomas et al. (2017) Performing Skin Microbiome Research: A Method to the Madness. J Invest Dermatol 137:561-568
Kong, Heidi H; Morris, Alison (2017) The emerging importance and challenges of the human mycobiome. Virulence 8:310-312
Byrd, Allyson L; Deming, Clay; Cassidy, Sara K B et al. (2017) Staphylococcus aureus and Staphylococcus epidermidis strain diversity underlying pediatric atopic dermatitis. Sci Transl Med 9:
Tsai, Yu-Chih; Conlan, Sean; Deming, Clayton et al. (2016) Resolving the Complexity of Human Skin Metagenomes Using Single-Molecule Sequencing. MBio 7:e01948-15
Bosch, Thomas C G; Zengler, Karsten; Naik, Shruti et al. (2016) FRT - FONDATION RENE TOURAINE: An International Foundation For Dermatology. Exp Dermatol 25:917-932
Kong, Heidi H (2016) Details Matter: Designing Skin Microbiome Studies. J Invest Dermatol 136:900-902
Oh, Julia; Byrd, Allyson L; Park, Morgan et al. (2016) Temporal Stability of the Human Skin Microbiome. Cell 165:854-66
Jo, Jay-Hyun; Kennedy, Elizabeth A; Kong, Heidi H (2016) Research Techniques Made Simple: Bacterial 16S Ribosomal RNA Gene Sequencing in Cutaneous Research. J Invest Dermatol 136:e23-e27

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