In this project we will transplant Nude/SCID mice with human bone marrow. Subsequently, we will transplant HLA matched various human cancers to variou sites. We will determine whether bone marrow derrived stem cells caarrying one reporter gene are migrating preferentially to areas of active cancers as in """"""""healing wound"""""""". Since both tumor cells and the stem cells will carry a different reporter gene, we will be able to determine whether fusion occured. If fusion occur to any clinical significance we will try to understand its mechanism, receptor and block it. We will also determine what makes normal bone marrow stem cells migrate into cancer and participate in carcinogenesis

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Investigator-Initiated Intramural Research Projects (ZIA)
Project #
1ZIABC011004-04
Application #
8349274
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
4
Fiscal Year
2011
Total Cost
$345,198
Indirect Cost
Name
National Cancer Institute Division of Basic Sciences
Department
Type
DUNS #
City
State
Country
Zip Code
Ray, Satyajit; Langan, Russell C; Mullinax, John E et al. (2012) Establishment of human ultra-low passage colorectal cancer cell lines using spheroids from fresh surgical specimens suitable for in vitro and in vivo studies. J Cancer 3:196-206
Mullinax, John E; Allins, Alexander; Avital, Itzhak (2011) Laparoscopic appendectomy for Amyand's hernia: a modern approach to a historic diagnosis. J Gastrointest Surg 15:533-5
Avital, Itzhak (2011) Regional chemotherapy for pancreatic cancer. J Surg Oncol 104:453