We have analyzed human centromeric chromatin containing CENPA from HeLa cells in culture using Chromatin IP followed by atomic force microscopy measurements and mass spectrophotometry. Thus far, we have identified that the CENPA chromatin structure is uniquely organized during interphase, and has fundamentally different characteristics from the rest of the chromatin which packages the genome. We plan to extend our analysis using live imaging coupled with AFM across the cell cycle to address how CENP-A is assembled and retained, as well as how it participates in non-centromeric functions at ectopic loci. We have discovered that CENP-A changes its structure over the cell cycle. This manuscript has been submitted for publication.

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Nuccio, A G; Bui, M; Dalal, Y et al. (2017) Mass Spectrometry-Based Methodology for Identification of Native Histone Variant Modifications From Mammalian Tissues and Solid Tumors. Methods Enzymol 586:275-290
Vlijm, R; Kim, S H; De Zwart, P L et al. (2017) The supercoiling state of DNA determines the handedness of both H3 and CENP-A nucleosomes. Nanoscale 9:1862-1870
Bui, Minh; Pitman, Mary; Nuccio, Arthur et al. (2017) Internal modifications in the CENP-A nucleosome modulate centromeric dynamics. Epigenetics Chromatin 10:17
Zhao, Haiqing; Winogradoff, David; Bui, Minh et al. (2016) Promiscuous Histone Mis-Assembly Is Actively Prevented by Chaperones. J Am Chem Soc 138:13207-13218
Kim, Sung Hyun; Vlijm, Rifka; van der Torre, Jaco et al. (2016) CENP-A and H3 Nucleosomes Display a Similar Stability to Force-Mediated Disassembly. PLoS One 11:e0165078
Quénet, D; Sturgill, D; Dalal, Y (2016) Identifying Centromeric RNAs Involved in Histone Dynamics In Vivo. Methods Enzymol 573:445-66
Winogradoff, David; Zhao, Haiqing; Dalal, Yamini et al. (2015) Shearing of the CENP-A dimerization interface mediates plasticity in the octameric centromeric nucleosome. Sci Rep 5:17038
Quénet, Delphine; Dalal, Yamini (2015) Correction: a long non-coding RNA is required for targeting centromeric protein A to the human centromere. Elife 4:
Walkiewicz, Marcin P; Dimitriadis, Emilios K; Dalal, Yamini (2014) CENP-A octamers do not confer a reduction in nucleosome height by AFM. Nat Struct Mol Biol 21:2-3
Dalal, Yamini; McNally, James G (2014) Now you see it, now you don't: a biochemist's primer for advances in high-resolution microscopy: comment on ""Biomolecular dynamics and binding studies in the living cell"" by Stephan Diekmann and Christian Hoischen. Phys Life Rev 11:36-7; discussion 38

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