The NHLBI Light Microscopy Core Facility (LMCF) facility has been in operation for nine years. The LMCF consists of two people, Dr. Christian A. Combs and Dr. Daniela A. Malide, and nine microscopes located in three locations. To date we have helped researchers publish more than 125 papers and assisted almost every NHLBI-DIR Center and Branch in the past nine years. In 2008-2009 we had our most productive years to date with over 24 papers published or in press. Research conducted in the LMCF has been on many disease states, basic cell biology, and on development and implementation of new imaging techniques. The general makeup of the LMCF reflects the evolving light microscopy needs of NHLBI-DIR researchers and a commitment to provide a maximal level of assistance to the NHLBI mission. The mission of this facility is to provide state-of-the-art equipment, training, and image processing capabilities to assist NHLBI-DIR researchers in experiments involving light microscopy. Researchers that work in this facility can expect support from core personnel to whatever level suits their research. This can include advanced microscopy techniques like two-photon microscopy to more ordinary wide-field imaging with a color camera Our emphasis is on training users to conduct the experiments themselves, although we are available for collaboration and all manner of assistance (experimental planning, data analysis and image processing, etc.) where required. Over the nine years this core has been in existence we have endeavored to provide a flexible and easy to use facility that meets researchers needs and allows them to conduct their experiments in an efficient manner even if they have had no prior microscopy experience. These goals are met in several ways. First, we have an array of microscopes that offer a wide-range of microscopy techniques. These microscopes and the microscopy techniques available have been chosen and developed in response to the specific needs of researchers in the institute. In addition to the microscopes, we provide a full suite of image processing programs a dedicated 64-bit image processing workstation. Where image-processing capabilities are lacking in these programs we either write our own image processing programs. Last, we provide a comprehensive web site about all facets of the core and microscopy instruction (http://dir-intranet.nhlbi.nih.gov/lmf/). The goal we are working towards with the website is to allow researchers a one-stop place to learn about the core and how to plan their experiments.

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1
Fiscal Year
2009
Total Cost
$566,673
Indirect Cost
Name
National Heart, Lung, and Blood Institute
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Dong, Fei; Jin, Xueting; Boettler, Michelle A et al. (2018) A Mouse Model of Schnyder Corneal Dystrophy with the N100S Point Mutation. Sci Rep 8:10219
Jin, Xueting; Dimitriadis, Emilios K; Liu, Ying et al. (2018) Macrophages Shed Excess Cholesterol in Unique Extracellular Structures Containing Cholesterol Microdomains. Arterioscler Thromb Vasc Biol 38:1504-1518
Glancy, Brian; Hartnell, Lisa M; Combs, Christian A et al. (2017) Power Grid Protection of the Muscle Mitochondrial Reticulum. Cell Rep 19:487-496
Combs, Christian A; Shroff, Hari (2017) Fluorescence Microscopy: A Concise Guide to Current Imaging Methods. Curr Protoc Neurosci 79:2.1.1-2.1.25
Sun, Nuo; Malide, Daniela; Liu, Jie et al. (2017) A fluorescence-based imaging method to measure in vitro and in vivo mitophagy using mt-Keima. Nat Protoc 12:1576-1587
Lucotte, Bertrand M; Powell, Chloe; Knutson, Jay R et al. (2017) Direct visualization of the arterial wall water permeability barrier using CARS microscopy. Proc Natl Acad Sci U S A 114:4805-4810
Billington, Neil; Beach, Jordan R; Heissler, Sarah M et al. (2015) Myosin 18A coassembles with nonmuscle myosin 2 to form mixed bipolar filaments. Curr Biol 25:942-8
Fredriksson, Karin; Van Itallie, Christina M; Aponte, Angel et al. (2015) Proteomic analysis of proteins surrounding occludin and claudin-4 reveals their proximity to signaling and trafficking networks. PLoS One 10:e0117074
Thacker, Seth G; Rousset, Xavier; Esmail, Safiya et al. (2015) Increased plasma cholesterol esterification by LCAT reduces diet-induced atherosclerosis in SR-BI knockout mice. J Lipid Res 56:1282-95
Glancy, Brian; Hartnell, Lisa M; Malide, Daniela et al. (2015) Mitochondrial reticulum for cellular energy distribution in muscle. Nature 523:617-20

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