The goal is to understand the virus-cell interactions that occur during reovirus infection of mammalian cells, with emphasis on determining those virus functions that are responsible for metabolic perturbations and eventual death of the host cell. Attention will be focussed on the proteins encoded by the reovirus S1 genome segment, o1, the virus cell attachment protein, and o1S(p14), whose function is presently unknown. The specific objectives are to determine the function(s) of sigms-1-5 in the replication cycle of reovirus and to investigate the possible involvement of sigma-1-S in the alteration of host cell metabolism. Antibodies have recently been prepared which react specifically to subcellular fractionation studies designed to determine the intracellular localization of sigma-1-s during reovirus multiplication. The interaction(s) of sigma-1-S with other reovirus and cellular proteins, or nucleic acids will also be determined. The proposed research will help determine the function of o1S and the effect of both sigma-1-S and sigma-1 on the host cell. This information will provide a more complete comprehension of the complex virus-host cell interactions that occur during virus multiplication.

Agency
National Science Foundation (NSF)
Institute
Division of Molecular and Cellular Biosciences (MCB)
Type
Standard Grant (Standard)
Application #
8918405
Program Officer
Maryanna P. Henkart
Project Start
Project End
Budget Start
1990-07-15
Budget End
1992-12-31
Support Year
Fiscal Year
1989
Total Cost
$100,000
Indirect Cost
Name
Medical College of Wisconsin
Department
Type
DUNS #
City
Milwaukee
State
WI
Country
United States
Zip Code
53226