Abstract

The core facility will consolidate all the morphological services for the use of the various projects. These services include histological preparations such as tissue processing, embedding sectioning and staining. Following histological processing the core will provide evaluation and interpretation of histopathological changes in tissue sections. These include evaluation and quantification of demyelination during coronavirus infection in mice, evaluation of virus-induced cerebrovascular disease in murine leukemia virus infection in mice, and analysis of HSV receptor expression in human brain. Computerized morphometric analysis will be offered as necessary. Additional morphological services or assistance will be provided for studies which require immunohistochemistry, immunofluorescence, in situ hybridization, in situ-PCR, and electron microscopy. Thus the core facility services are essential to the success of the fundamental goal of the project.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Research Program Projects (P01)
Project #
5P01NS030606-08
Application #
6324782
Study Section
Project Start
2000-07-01
Project End
2001-06-30
Budget Start
1998-10-01
Budget End
1999-09-30
Support Year
8
Fiscal Year
2000
Total Cost
$168,913
Indirect Cost

  Institution

Name
University of Pennsylvania
Department
Type
DUNS #
042250712
City
Philadelphia
State
PA
Country
United States
Zip Code
19104

  Publications

Plassmeyer, Matthew L; Soldan, Samantha S; Stachelek, Karen M et al. (2007) Mutagenesis of the La Crosse Virus glycoprotein supports a role for Gc (1066-1087) as the fusion peptide. Virology 358:273-82
Murphy, Samuel L; Chung-Landers, Maeran; Honczarenko, Marek et al. (2006) Linkage of reduced receptor affinity and superinfection to pathogenesis of TR1.3 murine leukemia virus. J Virol 80:4601-9
Simpson, Scott A; Manchak, Michael D; Hager, Elizabeth J et al. (2005) Nectin-1/HveC Mediates herpes simplex virus type 1 entry into primary human sensory neurons and fibroblasts. J Neurovirol 11:208-18
MacNamara, Katherine C; Chua, Ming Ming; Nelson, Peter T et al. (2005) Increased epitope-specific CD8+ T cells prevent murine coronavirus spread to the spinal cord and subsequent demyelination. J Virol 79:3370-81
Soldan, Samantha S; Plassmeyer, Matthew L; Matukonis, Meghan K et al. (2005) La Crosse virus nonstructural protein NSs counteracts the effects of short interfering RNA. J Virol 79:234-44
Weiss, Susan R; Navas-Martin, Sonia (2005) Coronavirus pathogenesis and the emerging pathogen severe acute respiratory syndrome coronavirus. Microbiol Mol Biol Rev 69:635-64
Plassmeyer, Matthew L; Soldan, Samantha S; Stachelek, Karen M et al. (2005) California serogroup Gc (G1) glycoprotein is the principal determinant of pH-dependent cell fusion and entry. Virology 338:121-32
Navas-Martin, Sonia R; Weiss, Susan (2004) Coronavirus replication and pathogenesis: Implications for the recent outbreak of severe acute respiratory syndrome (SARS), and the challenge for vaccine development. J Neurovirol 10:75-85
Fu, Li; Gonzales, Donna M; Das Sarma, Jayasri et al. (2004) A combination of mutations in the S1 part of the spike glycoprotein gene of coronavirus MHV-A59 abolishes demyelination. J Neurovirol 10:41-51
Landers, Maeran Chung; Dugger, Natalie; Quadros, Marlene et al. (2004) Neuropathogenic murine leukemia virus TR1.3 induces selective syncytia formation of brain capillary endothelium. Virology 321:57-64

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