Abstract

Fluorescence emission anisotropies, r, and mean lifetimes of 4-dimethylamino-omega-diphenylphosphinyl-trans-styrene (DDPS) were measured in n-butanol at different temperatures from 20 to 80xC for one- and two-photon excitation. When increasing the temperature, the lifetime decreased from 32 to 12 ps. The anisotropy values, however, remained constant (0.315 and 0.445 for one- and two-photon excitation, respectively) and were found to be temperature independent. For DDPS in glycerol at -5xC, where rotational diffusion does not occur during the excited state lifetime, the limiting emission anisotropies were r0 = 0.382 and r0 = 0.545 for one- and two-photon excitation, respectively, very little differing from the fundamental emission anisotropies rf = 2/5 and rf= 4/7. The small differences rf - r0 can be due to vibrations performed after excitation by the DDPS molecule as a result of the initial shock.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
5P41RR008119-09
Application #
6444711
Study Section
Project Start
2001-04-01
Project End
2002-03-31
Budget Start
1997-10-01
Budget End
1998-09-30
Support Year
9
Fiscal Year
2001
Total Cost
$293,069
Indirect Cost

  Institution

Name
University of Maryland Baltimore
Department
Type
DUNS #
003255213
City
Baltimore
State
MD
Country
United States
Zip Code
21201

  Publications

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Fu, Yi; Zhang, Jian; Lakowicz, Joseph R (2009) Highly efficient detection of single fluorophores in blood serum samples with high autofluorescence. Photochem Photobiol 85:646-51
Zhang, Jian; Badugu, Ramachandram; Lakowicz, Joseph R (2008) Fluorescence Quenching of CdTe Nanocrystals by Bound Gold Nanoparticles in Aqueous Solution. Plasmonics 3:3-11
Zhang, Jian; Lakowicz, Joseph R (2007) Metal-enhanced fluorescence of an organic fluorophore using gold particles. Opt Express 15:2598-606
Fu, Yi; Lakowicz, Joseph R (2007) Single-Molecule Studies of Enhanced Fluorescence on Silver Island Films. Plasmonics 2:1-4
Ray, Krishanu; Szmacinski, Henryk; Enderlein, Joerg et al. (2007) Distance dependence of surface plasmon-coupled emission observed using Langmuir-Blodgett films. Appl Phys Lett 90:251116
Chowdhury, Mustafa H; Malyn, Stuart N; Aslan, Kadir et al. (2007) First Observation of Surface Plasmon-Coupled Chemiluminescence (SPCC). Chem Phys Lett 435:114-118
Zhang, Jian; Fu, Yi; Chowdhury, Mustafa H et al. (2007) Enhanced Forster Resonance Energy Transfer on Single Metal Particle. 2. Dependence on Donor-Acceptor Separation Distance, Particle Size, and Distance from Metal Surface. J Phys Chem C Nanomater Interfaces 111:11784-11792
Zhang, Jian; Fu, Yi; Lakowicz, Joseph R (2007) Luminescent images of single gold nanoparticles and their labeling on silica beads. Opt Express 15:13415-20

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