Core - Zebrafish

Abstract

The zebrafish core will provide efficient and safe care for wild-type and mutant zebrafish that are studied by the investigators in this proposal. Initially, Core B will provide centralized reagents, methods and technology to the investigators for study of particular gene expression in wild-type embryos. As an epistasis analysis of blood forming mutants is undertaken, the core will provide a diverse set of mutant embyros for study. The core will be supervised by the PI (Dr. Leonard Zon), in collaboration with a technician after year one. Dr. Zon has experience in zebrafish biology. The zebrafish core may also be utilized to facilitate collaborations with other investigators in the Harvard Medical area.

Funding Agency

• Agency: National Institute of Health (NIH)
• Institute: National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
• Type: Specialized Center (P50)
• Project #: 5P50DK049216-04S1
• Application #: 2845069
• Budget Start: 1996-10-01
• Budget End: 1997-09-30
• Support Year: 4
• Fiscal Year: 1997

Institution

• Name: Children's Hospital Boston
• DUNS #: 076593722
• City: Boston
• State: MA
• Country: United States
• Zip Code: 02115

Publications

Bauer, Daniel E; Orkin, Stuart H (2015) Hemoglobin switching's surprise: the versatile transcription factor BCL11A is a master repressor of fetal hemoglobin. Curr Opin Genet Dev 33:62-70

Kumar, Lalit; Chou, Janet; Yee, Christina S K et al. (2014) Leucine-rich repeat containing 8A (LRRC8A) is essential for T lymphocyte development and function. J Exp Med 211:929-42

Paik, Elizabeth J; Mahony, Shaun; White, Richard M et al. (2013) A Cdx4-Sall4 regulatory module controls the transition from mesoderm formation to embryonic hematopoiesis. Stem Cell Reports 1:425-36

Boatman, Sonja; Barrett, Francesca; Satishchandran, Sruthi et al. (2013) Assaying hematopoiesis using zebrafish. Blood Cells Mol Dis 51:271-6

Deshpande, Aniruddha J; Chen, Liying; Fazio, Maurizio et al. (2013) Leukemic transformation by the MLL-AF6 fusion oncogene requires the H3K79 methyltransferase Dot1l. Blood 121:2533-41

Jing, Lili; Durand, Ellen M; Ezzio, Catherine et al. (2012) In situ hybridization assay-based small molecule screening in zebrafish. Curr Protoc Chem Biol 4:

Kalaitzidis, Demetrios; Sykes, Stephen M; Wang, Zhu et al. (2012) mTOR complex 1 plays critical roles in hematopoiesis and Pten-loss-evoked leukemogenesis. Cell Stem Cell 11:429-39

Alexander, Matthew S; Kawahara, Genri; Kho, Alvin T et al. (2011) Isolation and transcriptome analysis of adult zebrafish cells enriched for skeletal muscle progenitors. Muscle Nerve 43:741-50

Baffour-Awuah, Nana Yaa; Delemarre, Eveline; Fujiwara, Yuko et al. (2011) Characterization of expression in mice of a transgene containing 3.3 kb of the human lactase-phlorizin hydrolase (LPH) 5' flanking sequence. Dig Dis Sci 56:59-69

Guyon, Jeffrey R; Mosley, Alycia N; Jun, Susan J et al. (2005) Delta-sarcoglycan is required for early zebrafish muscle organization. Exp Cell Res 304:105-15