The aims of the proposd work are to investigate the roles of covalent enzyme phosphorylation of hepatic lipogenesis and the activity of the key rate-limiting enzyme of fatty acid synthesis, acetyl-CoA carboxylase. Particular emphasis is being placed on the characterization of site-specific phosphorylation changes in the enzyme indued by hormones in the intact cell and the characterization in vitro of the major site-specific protein kinases and phosphatases responsible for these alterations. In addition, the regulation of hepatic lipogenesis by insulin is being employed as a model system for the further elucidation of the molecular mechanism of insulin action..
| Glynn, B P; Colliton, J W; McDermott, J M et al. (1986) Phorbol esters, but not insulin, promote depletion of cytosolic protein kinase C in rat adipocytes. Biochem Biophys Res Commun 135:1119-25 |
| Witters, L A; McDermott, J M (1986) Regulation of acetyl-CoA carboxylase by ADP-ribosylation. Biochemistry 25:7216-20 |
| Blackshear, P J; Wen, L; Nemenoff, R A et al. (1986) Growth factor activation of protein kinase C-dependent and -independent pathways of protein phosphorylation in fibroblasts: relevance to activation of protein kinase C in neuronal tissues. Prog Brain Res 69:183-95 |
| Glynn, B; Colliton, J; McDermott, J et al. (1985) Assay of protein kinase C with an N-bromosuccinimide-cleavage fragment of histone H1. Biochem J 231:489-92 |
