Abstract

The overall goal of this research program is to reveal developmental processes in primate retina that shape its complex topography. Previous results have provided the first detailed description of foveal development in humans and monkeys. In the present application, it is proposed to investigate the developmental mechanisms of photoreceptors, amacrine and bipolar neurons using both in vivo descriptive and in vitro experimental approaches.
Aim 1 is to complete studies of the spatial and temporal sequence of cone and rod opsin mRNA/protein appearance and identify endogenous or exogenous factors that change the ratio or numbers of cone subtypes and rods.
Aim 2 is to complete studies to determine the temporal and spatial patterns of synapse formation related to cellular birthdates.
Aim 3 is to identify molecules which specify foveal address, support the maintenance or destruction of cell populations in the fovea or lead to the exclusion of other cell populations.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Eye Institute (NEI)
Type
Research Project (R01)
Project #
5R01EY004536-16
Application #
2888135
Study Section
Special Emphasis Panel (ZRG1-VISC (01))
Project Start
1983-03-01
Project End
2001-06-30
Budget Start
1999-07-01
Budget End
2001-06-30
Support Year
16
Fiscal Year
1999
Total Cost
Indirect Cost

  Institution

Name
University of Washington
Department
Anatomy/Cell Biology
Type
Schools of Medicine
DUNS #
135646524
City
Seattle
State
WA
Country
United States
Zip Code
98195

  Publications

Springer, A D; Hendrickson, A E (2005) Development of the primate area of high acuity, 3: temporal relationships between pit formation, retinal elongation and cone packing. Vis Neurosci 22:171-85
Bumsted O'Brien, Keely M; Cheng, Hong; Jiang, Yibin et al. (2004) Expression of photoreceptor-specific nuclear receptor NR2E3 in rod photoreceptors of fetal human retina. Invest Ophthalmol Vis Sci 45:2807-12
Springer, A D; Hendrickson, A E (2004) Development of the primate area of high acuity. 2. Quantitative morphological changes associated with retinal and pars plana growth. Vis Neurosci 21:775-90
Springer, A D; Hendrickson, A E (2004) Development of the primate area of high acuity. 1. Use of finite element analysis models to identify mechanical variables affecting pit formation. Vis Neurosci 21:53-62
Cornish, Elisa E; Xiao, Ming; Yang, Zhantao et al. (2004) The role of opsin expression and apoptosis in determination of cone types in human retina. Exp Eye Res 78:1143-54
Sandercoe, Trent M; Geller, Scott F; Hendrickson, Anita E et al. (2003) VEGF expression by ganglion cells in central retina before formation of the foveal depression in monkey retina: evidence of developmental hypoxia. J Comp Neurol 462:42-54
Fischer, A J; Hendrickson, A; Reh, T A (2001) Immunocytochemical characterization of cysts in the peripheral retina and pars plana of the adult primate. Invest Ophthalmol Vis Sci 42:3256-63
Swain, P K; Hicks, D; Mears, A J et al. (2001) Multiple phosphorylated isoforms of NRL are expressed in rod photoreceptors. J Biol Chem 276:36824-30
Milam, A H; Hendrickson, A E; Xiao, M et al. (2000) Localization of tubby-like protein 1 in developing and adult human retinas. Invest Ophthalmol Vis Sci 41:2352-6
Sears, S; Erickson, A; Hendrickson, A (2000) The spatial and temporal expression of outer segment proteins during development of Macaca monkey cones. Invest Ophthalmol Vis Sci 41:971-9

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