This project focuses on basic and applied research on human retroviruses, HIV-1 and HTLV-I. It covers three broad areas--I) basic research studying the regulated expression of HIV-1; II) basic research on cellular transformation events as related to HTLV-I; and III) applied research towards developing HIV-1-based vectors and designing molecular ribozymes targeted against HIV-1. Some notable scientific findings from our research program in 1997-1998 include: 1) the demonstration that Tat binds a histone acetylase and affects transcription of integrated LTR-promoters; 2) evidence that Tat can influence shut-off host cell protein synthesis by binding to EF-1d; 3) evidence that extracellular Tat protein antagonize CXCR4-receptor usage; 4) generation of an attenuated HIV-1 virus using IkB-a; 5) the characterization of mitotic checkpoint proteins HsMAD1 and HsMAD2 and their interactions with HTLV-I Tax in cellular transformation; 6) demonstration of Tax effects on G1 to S cell phase transition through modulation of cyclin D-cdk and p110RB; 7) the identification of a novel selenium containing protein; and 8) the generation of infectious HIV viral particles containing a therapeutic ribozyme. In the coming year, we plan to extend our findings into each of these research areas.
| Yeung, Man Lung; Houzet, Laurent; Yedavalli, Venkat S R K et al. (2009) A genome-wide short hairpin RNA screening of jurkat T-cells for human proteins contributing to productive HIV-1 replication. J Biol Chem 284:19463-73 |
| Yeung, Man Lung; Bennasser, Yamina; Watashi, Koichi et al. (2009) Pyrosequencing of small non-coding RNAs in HIV-1 infected cells: evidence for the processing of a viral-cellular double-stranded RNA hybrid. Nucleic Acids Res 37:6575-86 |
| Grassmann, Ralph; Jeang, Kuan-Teh (2008) The roles of microRNAs in mammalian virus infection. Biochim Biophys Acta : |
| Berkhout, Ben; Jeang, Kuan-Teh (2007) RISCy business: MicroRNAs, pathogenesis, and viruses. J Biol Chem 282:26641-5 |
| Yeung, Man Lung; Bennasser, Yamina; Le, Shu-Yun et al. (2007) RNA interference and HIV-1. Adv Pharmacol 55:427-38 |
| Yeung, Man Lung; Benkirane, Monsef; Jeang, Kuan-Teh (2007) Small non-coding RNAs, mammalian cells, and viruses: regulatory interactions? Retrovirology 4:74 |
| Yedavalli, Venkat R K; Jeang, Kuan-Teh (2007) Methylation: a regulator of HIV-1 replication? Retrovirology 4:9 |
| Triboulet, Robinson; Mari, Bernard; Lin, Yea-Lih et al. (2007) Suppression of microRNA-silencing pathway by HIV-1 during virus replication. Science 315:1579-82 |
| Bennasser, Yamina; Yeung, Man Lung; Jeang, Kuan-Teh (2007) RNAi therapy for HIV infection: principles and practicalities. BioDrugs 21:17-22 |
| Yeung, Man Lung; Bennasser, Yamina; Jeang, Kuan-Teh (2007) miRNAs in the biology of cancers and viral infections. Curr Med Chem 14:191-7 |
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