Abstract

The human immunodeficiency virus (HIV-1) is a complex retrovirus with regard to its genome architecture and gene expression. The human retroviruses including HIV-1 code for specific viral proteins which regulate gene expression at the transcriptional and post-transcriptional levels. The transactivator of HIV transcription (Tat) is one such protein, which has been the subject of intense investigation. The first objective of this pilot project s the development and application lf RNA viral vectors to study the molecular mechanism of Tat protein function. RNA viral vectors represent a new generation of biomolecules which are being exploited to deliver specific RNA viral molecules into cells. The second objective of this pilot project involves the construction of RNA vectors carrying TAT specific antisense and decoy RNA sequences of HIV-1. The constructs will be used to inhibit the HIV-1 gene expression in tissue culture systems. These two objectives are interlinked, and involve very similar experimental strategies.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Research Centers in Minority Institutions Award (G12)
Project #
5G12RR003032-15
Application #
6205928
Study Section
Project Start
1999-09-30
Project End
2000-09-29
Budget Start
1997-10-01
Budget End
1998-09-30
Support Year
15
Fiscal Year
1999
Total Cost
Indirect Cost

  Institution

Name
Meharry Medical College
Department
Type
DUNS #
City
Nashville
State
TN
Country
United States
Zip Code
37208

  Publications

Popik, Waldemar; Khatua, Atanu; Hildreth, James E K et al. (2018) Phosphorodiamidate morpholino targeting the 5' untranslated region of the ZIKV RNA inhibits virus replication. Virology 519:77-85
Choudhury, Natasha A; DeBaun, Michael R; Ponisio, Maria R et al. (2018) Intracranial vasculopathy and infarct recurrence in children with sickle cell anaemia, silent cerebral infarcts and normal transcranial Doppler velocities. Br J Haematol 183:324-326
Ogunsile, Foluso J; Currie, Kelli L; Rodeghier, Mark et al. (2018) History of parvovirus B19 infection is associated with silent cerebral infarcts. Pediatr Blood Cancer 65:
Abney, Kristopher K; Ramos-Hunter, Susan J; Romaine, Ian M et al. (2018) Selective Activation of N,N'-Diacyl Rhodamine Pro-fluorophores Paired with Releasing Enzyme, Porcine Liver Esterase (PLE). Chemistry 24:8985-8988
Al-Hendy, Ayman; Laknaur, Archana; Diamond, Michael P et al. (2017) Silencing Med12 Gene Reduces Proliferation of Human Leiomyoma Cells Mediated via Wnt/?-Catenin Signaling Pathway. Endocrinology 158:592-603
Heerman, William J; Jackson, Natalie; Roumie, Christianne L et al. (2017) Recruitment methods for survey research: Findings from the Mid-South Clinical Data Research Network. Contemp Clin Trials 62:50-55
Kenerson, Donna; Fadeyi, Saudat; Liu, Jianguo et al. (2017) Processes in Increasing Participation of African American Women in Cancer Prevention Trials: Development and Pretesting of an Audio-Card. J Health Commun 22:933-941
Yang, Seung Woo; Cho, Eun Hee; Choi, So Young et al. (2017) DC-SIGN expression in Hofbauer cells may play an important role in immune tolerance in fetal chorionic villi during the development of preeclampsia. J Reprod Immunol 124:30-37
Erves, Jennifer Cunningham; Mayo-Gamble, Tilicia L; Hull, Pamela C et al. (2017) Erratum to: Adolescent Participation in HPV Vaccine Clinical Trials: Are Parents Willing? J Community Health 42:902
Mouton, Charles P; Southerland, Janet H (2017) Elder Abuse in the African Diaspora: A Review. J Natl Med Assoc 109:262-271

Showing the most recent 10 out of 225 publications