The overall objective of this proposal is to study the host/pathogen interaction at mucosal surfaces and to develop prevention strategies that maximize protective mucosal immune responses to Cryptosporidiumum parvum (C parvum). We will develop and maintain a murine model for murine acquired immunodeficiency syndrome (MAIDS) which will be used to study the pathogenesis of C parmm; a potential pathogen commonly associated with AIDS. To develop the MAIDS model, C57BI_16 female mice win be immunosuppressed by inoculation with LP-BM5; then challenged with C parvum 3 months post LP-BM5 infection. Studies of experimentally induced cryptosporidiosis using this model, will serve as a relevant tool for evaluating various therapies for prevention of this opportunistic disease as our previous studies have confirmed that mice infected with LP-BM5, develop persistent experimental cryptosporidiosis with high numbers of oocysts shed in the feces. Since the spread of AIDS is global and frequently assoc iated with opportunistic infections including cryptosporidiosis, it is critical to control AIDS-related o0portunistic diseases to alleviate human suffering in order to minimize both social and economic impact on society. Our long range goal will be to develop effective prophylactic regimens for the control of Cryptosporidium. infection, especially through nutritional, immunotherapeutic or chemotherapeutic interventions. As yet, no effective treatment for cryptosporidiosis has been reported. We will achieve the following specific aims during these studies: elucidate the role of cellular gut immunity to C parvum in this MAIDS model by determining the roles and phenotypic frequencies of intestinal (1) intraepithelial (EEL's) and (2) lamina propria (LPL) subpopulations (CD4', CD8, IgA, IgG' and IgM') and cytokine (TNF-a , IFN-y, IL-1, IL-2, 4, 5 and 10) production post C parvum challenge. (3) evaluate the efficacy of Lactobacillus reuteri and L. acidophilus as probiotics for the control of cryptosporidiosis and (4) evaluate the efficacy of probiotics and (5) vavvines administered simultaneously for the control of cryptosporidiosis. Results obtained from these studies will be relevant in the development of new therapies for the control of cryptosporidiosis and other opportunistic diseases in immuncompromised individuals especially AIDS subjects.

Project Start
1998-06-01
Project End
1999-05-31
Budget Start
1997-10-01
Budget End
1998-09-30
Support Year
11
Fiscal Year
1998
Total Cost
Indirect Cost
Name
Tuskegee University
Department
Type
DUNS #
128214178
City
Tuskegee
State
AL
Country
United States
Zip Code
36088
Mukherjee, Angana; Hollern, Daniel P; Williams, Oluwasina G et al. (2018) A Review of FOXI3 Regulation of Development and Possible Roles in Cancer Progression and Metastasis. Front Cell Dev Biol 6:69
Yates, Clayton; Long, Mark D; Campbell, Moray J et al. (2017) miRNAs as drivers of TMPRSS2-ERG negative prostate tumors in African American men. Front Biosci (Landmark Ed) 22:212-229
Chowdhury, Rupak; David, Nganwa; Bogale, Asseged et al. (2016) Assessing the Key Attributes of Low Utilization of Mammography Screening and Breast-self Exam among African-American Women. J Cancer 7:532-7
Jones, Jacqueline; Mukherjee, Angana; Karanam, Balasubramanyam et al. (2016) African Americans with pancreatic ductal adenocarcinoma exhibit gender differences in Kaiso expression. Cancer Lett 380:513-22
Wang, Honghe; Liu, Wei; Black, ShaNekkia et al. (2016) Kaiso, a transcriptional repressor, promotes cell migration and invasion of prostate cancer cells through regulation of miR-31 expression. Oncotarget 7:5677-89
Reams, R Renee; Jones-Triche, Jacqueline; Chan, Owen T M et al. (2015) Immunohistological analysis of ABCD3 expression in Caucasian and African American prostate tumors. Biomed Res Int 2015:132981
Arora, Ritu; Schmitt, David; Karanam, Balasubramanyam et al. (2015) Inhibition of the Warburg effect with a natural compound reveals a novel measurement for determining the metastatic potential of breast cancers. Oncotarget 6:662-78
Jones, Jacqueline; Wang, Honghe; Karanam, Balasubramanyam et al. (2014) Nuclear localization of Kaiso promotes the poorly differentiated phenotype and EMT in infiltrating ductal carcinomas. Clin Exp Metastasis 31:497-510
Okumu, Lilian A; Braden, Tim D; Vail, Krystal et al. (2014) Low androgen induced penile maldevelopment involves altered gene expression of biomarkers of smooth muscle differentiation and a key enzyme regulating cavernous smooth muscle cell tone. J Urol 192:267-73
Theodore, Shaniece C; Davis, Melissa; Zhao, Fu et al. (2014) MicroRNA profiling of novel African American and Caucasian Prostate Cancer cell lines reveals a reciprocal regulatory relationship of miR-152 and DNA methyltranferase 1. Oncotarget 5:3512-25

Showing the most recent 10 out of 101 publications