Contig Maps of Human Chromosome 17q22q23

Shah, Ketan S.

Abstract

The specific goal is to complete construction of the YAC contig map for about 15 cM sex-average region within the 17q22-q23 (at D17S86 locus: MPO-GH1 region).
The specific aims are: (a) define the order of 12 microsatellite markers and size of all (about 25) YACs representing 17q22-q23 region by inter Alu-PCR fingerprinting and Pulsed Field Gel Electrophoresis (PFGE), (b) complete the gaps between different markers by (I) employing one of the techniques e.g. """"""""vectorette""""""""-PCR; to amplify small end-specific fragments of the human DNA insert from the appropriate YACs, (ii) cycle sequence the verified fragment by an enzymatic method using commercially available kits and an automated DNA sequencer, (iii) design PCR primers that are unique for the fragment (Sequence-Tagged-Site; STS) using software e.g. PRIMER or DNASIS, and test the primers on human DNA template, (iv) employ the STSs to obtain overlapping YAC clone(s) from the Michigan Genome Center or CEPH (Centre d'Etude du Polymorphisme Humain), France or through commercial service such as Research Genetics. Other available internal probes such as pC63, pTHH59, pTh17.12, 128E1 and pRMU1 will also be utilized to define more STSs in 17q22-q23 region.

Funding Agency

• Agency: National Institute of Health (NIH)
• Institute: National Center for Research Resources (NCRR)
• Type: Research Centers in Minority Institutions Award (G12)
• Project #: 5G12RR003062-12
• Application #: 3723804
• Support Year: 12
• Fiscal Year: 1996

Institution

• Name: Clark Atlanta University
• DUNS #: 065325177
• City: Atlanta
• State: GA
• Country: United States
• Zip Code: 30314

Publications

Morton, Derrick J; Patel, Divya; Joshi, Jugal et al. (2017) ID4 regulates transcriptional activity of wild type and mutant p53 via K373 acetylation. Oncotarget 8:2536-2549

Joshi, Jugal Bharat; Patel, Divya; Morton, Derrick J et al. (2017) Inactivation of ID4 promotes a CRPC phenotype with constitutive AR activation through FKBP52. Mol Oncol 11:337-357

Wilder, Catera L; Walton, Charlene; Watson, Valencia et al. (2016) Differential cathepsin responses to inhibitor-induced feedback: E-64 and cystatin C elevate active cathepsin S and suppress active cathepsin L in breast cancer cells. Int J Biochem Cell Biol 79:199-208

Komaragiri, Shravan Kumar; Bostanthirige, Dhanushka H; Morton, Derrick J et al. (2016) ID4 promotes AR expression and blocks tumorigenicity of PC3 prostate cancer cells. Biochem Biophys Res Commun 478:60-66

Brown, Shanora G; Knowell, Ashley E; Hunt, Aisha et al. (2015) Interferon inducible antiviral MxA is inversely associated with prostate cancer and regulates cell cycle, invasion and Docetaxel induced apoptosis. Prostate 75:266-79

Muniyan, Sakthivel; Chou, Yu-Wei; Tsai, Te-Jung et al. (2015) p66Shc longevity protein regulates the proliferation of human ovarian cancer cells. Mol Carcinog 54:618-31

Chinaranagari, Swathi; Sharma, Pankaj; Bowen, Nathan J et al. (2015) Prostate cancer epigenome. Methods Mol Biol 1238:125-40

Burton, Liza J; Smith, Basil A; Smith, Bethany N et al. (2015) Muscadine grape skin extract can antagonize Snail-cathepsin L-mediated invasion, migration and osteoclastogenesis in prostate and breast cancer cells. Carcinogenesis 36:1019-27

Goodson 3rd, William H; Lowe, Leroy; Carpenter, David O et al. (2015) Assessing the carcinogenic potential of low-dose exposures to chemical mixtures in the environment: the challenge ahead. Carcinogenesis 36 Suppl 1:S254-96

Mandal, S; Abebe, F; Chaudhary, J (2014) -174G/C polymorphism in the interleukin-6 promoter is differently associated with prostate cancer incidence depending on race. Genet Mol Res 13:139-51