This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The University of Texas at El Paso proposes continuing support for a multi-user research facility specializing in the separation and characterization of biomolecules, the molecular biology core facility. This facility maintains equipment such as centrifuges, ultracentrifuges, gamma and scintillation counters, HPLC with numerous detectors, capillary electrophoresis, UV/vis spectrophotometers, microplate readers and many other items of shared equipment. Numerous other resources are available as well to document and analyze experimental data collected using these instruments. Currently, this core is also equipped for the synthesis and amplification of nucleic acids. These capabilities may be reorganized to better serve the needs of users as a result of possible relocation and discussions with affected user groups. The purpose of this facility is to provide the broadest possible group of users with access to well maintained, state of the art, equipment. Each of two rooms housing these instruments is entered more than 1000 times per month by more than 100 individual users, representing more than 15 research groups. This facility will be staffed by a full-time coordinator who will be responsible for routine maintenance, scheduling preventative maintenance by factory representatives, user orientation and assistance as well as account and inventory maintenance. Partial salary will be provided to the core director for ongoing oversight of these activities
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