My aims for research during the coming five years include a number of different projects. One has to do with the characterization of enkephalin convertase, a carboxypeptidase which appears to be associated with the biosynthesis of enkephalin. We will try to measure the turnover of the enzyme and evaluate in detail its localization. Another project relates to angiotensin converting enzyme which can be labeled with the inhibitor H-3-captopril. We propose to attempt to identify what seems to be a novel neuropeptide which is the endoyenous substrate for this enzyme. We propose to localize endogenous adenosine and ascertain its possible neutrotransmitter role. We will continue studies of calcium antagonist receptors and their relationship to calcium flux in neurons. We will characterize biogenic amine uptake sites with a variety of ligands. We propose studies to further elucidate the function of sigma receptors which are associated with the psychotomimetic effects of opiates. We propose detailed investigations of phorbol ester receptors to clarify their relationship to protein kinase C in neuronal function. We will also evaluate the relationship of the receptor interactions fo neutoxin MPTP with monamine oxiuase.

Agency
National Institute of Health (NIH)
Institute
National Institute on Drug Abuse (NIDA)
Type
Research Scientist Award (K05)
Project #
2K05DA000074-06
Application #
3075371
Study Section
(DABB)
Project Start
1980-07-01
Project End
1990-06-30
Budget Start
1985-07-01
Budget End
1986-06-30
Support Year
6
Fiscal Year
1985
Total Cost
Indirect Cost
Name
Johns Hopkins University
Department
Type
Schools of Medicine
DUNS #
045911138
City
Baltimore
State
MD
Country
United States
Zip Code
21218
Yuan, Guoxiang; Vasavda, Chirag; Peng, Ying-Jie et al. (2015) Protein kinase G-regulated production of H2S governs oxygen sensing. Sci Signal 8:ra37
Peng, Ying-Jie; Makarenko, Vladislav V; Nanduri, Jayasri et al. (2014) Inherent variations in CO-H2S-mediated carotid body O2 sensing mediate hypertension and pulmonary edema. Proc Natl Acad Sci U S A 111:1174-9
Bang, Sookhee; Kim, Seyun; Dailey, Megan J et al. (2012) AMP-activated protein kinase is physiologically regulated by inositol polyphosphate multikinase. Proc Natl Acad Sci U S A 109:616-20
Dailey, Megan J; Kim, Seyun (2012) Inositol polyphosphate multikinase: an emerging player for the central action of AMP-activated protein kinase. Biochem Biophys Res Commun 421:1-3
Kim, Seyun; Kim, Sangwon F; Maag, David et al. (2011) Amino acid signaling to mTOR mediated by inositol polyphosphate multikinase. Cell Metab 13:215-21
Maag, David; Maxwell, Micah J; Hardesty, Douglas A et al. (2011) Inositol polyphosphate multikinase is a physiologic PI3-kinase that activates Akt/PKB. Proc Natl Acad Sci U S A 108:1391-6
Ho, Gary P H; Selvakumar, Balakrishnan; Mukai, Jun et al. (2011) S-nitrosylation and S-palmitoylation reciprocally regulate synaptic targeting of PSD-95. Neuron 71:131-41
Mustafa, Asif K; Sikka, Gautam; Gazi, Sadia K et al. (2011) Hydrogen sulfide as endothelium-derived hyperpolarizing factor sulfhydrates potassium channels. Circ Res 109:1259-68
Sen, Nilkantha; Snyder, Solomon H (2010) Protein modifications involved in neurotransmitter and gasotransmitter signaling. Trends Neurosci 33:493-502
Paul, B D; Snyder, S H (2010) The unusual amino acid L-ergothioneine is a physiologic cytoprotectant. Cell Death Differ 17:1134-40

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