Abstract

Using a known rat lung cDNA sequence, specific sets of primers are designed for a polymerase chain reaction (PCR) to amplify fragments of VIP receptor from a rat genomic DNA. These fragments are subcloned to make a radioactive probe to screen mRNA expression in the rat sublingual salivary gland (RSLG) and to close a complete cDNA copy from a RSLG cDNA library. After isolating a VIP receptor cDNA, the expression of cDNA and its role in signal transduction will be further investigated. This study will help to elucidate the complete pathway of salivary secretion in sublingual gland cells. KEY WORDS: cDNA, mRNA, rat sublingual gland, salivary secretion

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Dental & Craniofacial Research (NIDCR)
Type
Unknown (K16)
Project #
5K16DE000159-10
Application #
3753454
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
10
Fiscal Year
1994
Total Cost
Indirect Cost

  Institution

Name
University of Rochester
Department
Type
DUNS #
208469486
City
Rochester
State
NY
Country
United States
Zip Code
14627

  Publications

Howard, E W; Newman, L A; Oleksyn, D W et al. (2001) SpKrl: a direct target of beta-catenin regulation required for endoderm differentiation in sea urchin embryos. Development 128:365-75
Caldwell, C E; Marquis, R E (1999) Oxygen metabolism by Treponema denticola. Oral Microbiol Immunol 14:66-72
New, D R; Ma, M; Epstein, L G et al. (1997) Human immunodeficiency virus type 1 Tat protein induces death by apoptosis in primary human neuron cultures. J Neurovirol 3:168-73
Kaplan, M D; Olschowka, J A; O'Banion, M K (1997) Cyclooxygenase-1 behaves as a delayed response gene in PC12 cells differentiated by nerve growth factor. J Biol Chem 272:18534-7
O'Banion, M K; Miller, J C; Chang, J W et al. (1996) Interleukin-1 beta induces prostaglandin G/H synthase-2 (cyclooxygenase-2) in primary murine astrocyte cultures. J Neurochem 66:2532-40
Madden, T E; Clark, V L; Kuramitsu, H K (1995) Revised sequence of the Porphyromonas gingivalis prtT cysteine protease/hemagglutinin gene: homology with streptococcal pyrogenic exotoxin B/streptococcal proteinase. Infect Immun 63:238-47
O'Connell, B C; Tabak, L A (1993) Separation of glycopeptides from in vitro O-glycosylation reactions using C18 cartridges. Anal Biochem 210:423-5
O'Connell, B C; Tabak, L A (1993) A comparison of serine and threonine O-glycosylation by UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase. J Dent Res 72:1554-8
O'Connell, B C; Hagen, F K; Tabak, L A (1992) The influence of flanking sequence on the O-glycosylation of threonine in vitro. J Biol Chem 267:25010-8
Madden, T E; Thompson, T M; Clark, V L (1992) Expression of Porphyromonas gingivalis proteolytic activity in Escherichia coli. Oral Microbiol Immunol 7:349-56

Showing the most recent 10 out of 22 publications