Abstract

In the parotid gland acini cells as in many other secretory cells, the salt and fluid secretion is dependent on the transepithelial chloride transport. Our lab has recently discovered three different types of chloride channels in the parotid acini cells - voltage activated, volume activated, and calcium activated - requiring the characterization of functional roles of each type to understand better the physiology of saliva secretion.
My specific aim of this project is as follows: 1) I will study how secretory stimulation effects the gating open probability) of each channel temporally in relationship to the intracellular calcium and membrane potential. 2) I will study spatial distribution of three types of channel to correlate their cellular location and the physiological function. 3) I will attempt to modify current models of salt and fluid secretion to build a new analytical model incorporating the three types of chloride channels, to explain clearly the phenomena of salivary secretion and also to guide and direct further study.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Dental & Craniofacial Research (NIDCR)
Type
Unknown (K16)
Project #
2K16DE000159-11
Application #
5210023
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
11
Fiscal Year
1996
Total Cost
Indirect Cost

  Publications

Howard, E W; Newman, L A; Oleksyn, D W et al. (2001) SpKrl: a direct target of beta-catenin regulation required for endoderm differentiation in sea urchin embryos. Development 128:365-75
Caldwell, C E; Marquis, R E (1999) Oxygen metabolism by Treponema denticola. Oral Microbiol Immunol 14:66-72
New, D R; Ma, M; Epstein, L G et al. (1997) Human immunodeficiency virus type 1 Tat protein induces death by apoptosis in primary human neuron cultures. J Neurovirol 3:168-73
Kaplan, M D; Olschowka, J A; O'Banion, M K (1997) Cyclooxygenase-1 behaves as a delayed response gene in PC12 cells differentiated by nerve growth factor. J Biol Chem 272:18534-7
O'Banion, M K; Miller, J C; Chang, J W et al. (1996) Interleukin-1 beta induces prostaglandin G/H synthase-2 (cyclooxygenase-2) in primary murine astrocyte cultures. J Neurochem 66:2532-40
Madden, T E; Clark, V L; Kuramitsu, H K (1995) Revised sequence of the Porphyromonas gingivalis prtT cysteine protease/hemagglutinin gene: homology with streptococcal pyrogenic exotoxin B/streptococcal proteinase. Infect Immun 63:238-47
O'Connell, B C; Tabak, L A (1993) Separation of glycopeptides from in vitro O-glycosylation reactions using C18 cartridges. Anal Biochem 210:423-5
O'Connell, B C; Tabak, L A (1993) A comparison of serine and threonine O-glycosylation by UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase. J Dent Res 72:1554-8
O'Connell, B C; Hagen, F K; Tabak, L A (1992) The influence of flanking sequence on the O-glycosylation of threonine in vitro. J Biol Chem 267:25010-8
Madden, T E; Thompson, T M; Clark, V L (1992) Expression of Porphyromonas gingivalis proteolytic activity in Escherichia coli. Oral Microbiol Immunol 7:349-56

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