Abstract

Cyclooxygenase is known to be important in physiologic functions such as inflammation and pain modulation. Recently, an inducible form of this enzyme, Cyclooxygenase)2 (COX-2),was discovered. COX-2 induced by inflammatory mediators such as IL)lB, as well as agents known to activate protein kinase-C (i.e. phorbol esters like TPA), possibly through NF)kB. To better understand the mechanisms of prostaglandlin production in the CNS, we have examined COX-2 activity and expression in primary astrocytes cultures. We demonstrate synergistic increase in prostaglandlin accumulation and COX-2 activity with the application of lL)1B and TPA, and postulate that these agents are working through a common pathway mediated by NF)kB.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Dental & Craniofacial Research (NIDCR)
Type
Unknown (K16)
Project #
2K16DE000159-11
Application #
5210028
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
11
Fiscal Year
1996
Total Cost
Indirect Cost

  Publications

Howard, E W; Newman, L A; Oleksyn, D W et al. (2001) SpKrl: a direct target of beta-catenin regulation required for endoderm differentiation in sea urchin embryos. Development 128:365-75
Caldwell, C E; Marquis, R E (1999) Oxygen metabolism by Treponema denticola. Oral Microbiol Immunol 14:66-72
New, D R; Ma, M; Epstein, L G et al. (1997) Human immunodeficiency virus type 1 Tat protein induces death by apoptosis in primary human neuron cultures. J Neurovirol 3:168-73
Kaplan, M D; Olschowka, J A; O'Banion, M K (1997) Cyclooxygenase-1 behaves as a delayed response gene in PC12 cells differentiated by nerve growth factor. J Biol Chem 272:18534-7
O'Banion, M K; Miller, J C; Chang, J W et al. (1996) Interleukin-1 beta induces prostaglandin G/H synthase-2 (cyclooxygenase-2) in primary murine astrocyte cultures. J Neurochem 66:2532-40
Madden, T E; Clark, V L; Kuramitsu, H K (1995) Revised sequence of the Porphyromonas gingivalis prtT cysteine protease/hemagglutinin gene: homology with streptococcal pyrogenic exotoxin B/streptococcal proteinase. Infect Immun 63:238-47
O'Connell, B C; Tabak, L A (1993) Separation of glycopeptides from in vitro O-glycosylation reactions using C18 cartridges. Anal Biochem 210:423-5
O'Connell, B C; Tabak, L A (1993) A comparison of serine and threonine O-glycosylation by UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase. J Dent Res 72:1554-8
O'Connell, B C; Hagen, F K; Tabak, L A (1992) The influence of flanking sequence on the O-glycosylation of threonine in vitro. J Biol Chem 267:25010-8
Madden, T E; Thompson, T M; Clark, V L (1992) Expression of Porphyromonas gingivalis proteolytic activity in Escherichia coli. Oral Microbiol Immunol 7:349-56

Showing the most recent 10 out of 22 publications