Abstract

The purpose of this study was to determine the role of zALK-8, a novel type II serine/threonine kinase receptor, in crainofacial growth and development. To do so, DNA constructs of wild type, constitutively active, and truncated forms of this receptor were ligated into plasmid, transformed, and isolated via minipreps. 1-% agarose gels followed by sequence analysis confirmed isolation of these amplified constructs. mRNA from each construct is being employed in microinjection studies using the zebrafish as an animal model to help determine the role of this novel receptor in development. Potential ligand interactions can be predicted based on the phenotypic expression seen as the injected fish develop, however characterization and isolation of the actual ligand for this receptor is being determined through collaboration with investigators in France.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Dental & Craniofacial Research (NIDCR)
Type
Unknown (K16)
Project #
5K16DE000275-11
Application #
6474590
Study Section
Project Start
2001-07-01
Project End
2002-06-30
Budget Start
Budget End
Support Year
11
Fiscal Year
2001
Total Cost
$208,185
Indirect Cost

  Publications

McDermott, Nancy E; Chuang, Sung-Kiang; Woo, Valerie V et al. (2006) Maxillary sinus augmentation as a risk factor for implant failure. Int J Oral Maxillofac Implants 21:366-74
Chuang, S K; Cai, T; Douglass, C W et al. (2005) Frailty approach for the analysis of clustered failure time observations in dental research. J Dent Res 84:54-8
Chuang, S-K; Hatch, J P; Rugh, J et al. (2005) Multi-center randomized clinical trials in oral and maxillofacial surgery: modeling of fixed and random effects. Int J Oral Maxillofac Surg 34:341-4
Treister, Nathaniel S; Woo, Sook-Bin; O'Holleran, Eileen W et al. (2005) Oral chronic graft-versus-host disease in pediatric patients after hematopoietic stem cell transplantation. Biol Blood Marrow Transplant 11:721-31
Woo, Valerie V; Chuang, Sung-Kiang; Daher, Shadi et al. (2004) Dentoalveolar reconstructive procedures as a risk factor for implant failure. J Oral Maxillofac Surg 62:773-80
Halpern, Leslie R; Carter, Jeffrey B; Chuang, Sung-Kiang et al. (2003) A comparison of 2 consultation and treatment strategies to manage impacted third molars. J Oral Maxillofac Surg 61:779-84
Basile, John R; Eichten, Alexandra; Zacny, Valerie et al. (2003) NF-kappaB-mediated induction of p21(Cip1/Waf1) by tumor necrosis factor alpha induces growth arrest and cytoprotection in normal human keratinocytes. Mol Cancer Res 1:262-70
McDermott, Nancy E; Chuang, Sung-Kiang; Woo, Valerie V et al. (2003) Complications of dental implants: identification, frequency, and associated risk factors. Int J Oral Maxillofac Implants 18:848-55
Vehemente, Valerie A; Chuang, Sung-Kiang; Daher, Shadi et al. (2002) Risk factors affecting dental implant survival. J Oral Implantol 28:74-81
Chuang, S K; Tian, L; Wei, L J et al. (2002) Predicting dental implant survival by use of the marginal approach of the semi-parametric survival methods for clustered observations. J Dent Res 81:851-5

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