Over the past 18 yrs, my laboratory has been investigating human IgE immunoregulation through an in vitro culture system. Our studies using in vitro cultures of blood MNC to study human IgE synthesis induced by recombinant human interleukin 4 (IL-4) have resulted in the development of a highly useful system. The proposed studies will continue to evaluate the usefulness of this system to investigate the regulation of IgE synthesis in normal and hyper IgE states, such as atopy, using IL-4, IL-13, IL-7, IL-10, IL-15, IL-12, IFN(, anti-CD40 and other reagents that have shown promise in this regard.These studies include: 1) the establishment of alloreactive, auto-reactive and antigen-specific T helper cell clones (some of which can trigger normal B cell IgE synthesis) and Epstein-Barr virus-transformed B cell lines which will be used to stimulate and maintain the T cell clones; 2) studies of normal and atopic B cell IgE synthesis when cultured with such clones or purified fresh T cells; with or without the addition of IL-4, IL-13, IL-7, IL-10, IL-15, IL-12, IFN(, anti-CD40 and other mAb's.
| Askie, Lisa M; Darlow, Brian A; Finer, Neil et al. (2018) Association Between Oxygen Saturation Targeting and Death or Disability in Extremely Preterm Infants in the Neonatal Oxygenation Prospective Meta-analysis Collaboration. JAMA 319:2190-2201 |
| Srinivasan, Lakshmi; Page, Grier; Kirpalani, Haresh et al. (2017) Genome-wide association study of sepsis in extremely premature infants. Arch Dis Child Fetal Neonatal Ed 102:F439-F445 |
| Denson, Lee A; McDonald, Scott A; Das, Abhik et al. (2017) Early Elevation in Interleukin-6 is Associated with Reduced Growth in Extremely Low Birth Weight Infants. Am J Perinatol 34:240-247 |
| James, Jennifer; Munson, David; DeMauro, Sara B et al. (2017) Outcomes of Preterm Infants following Discussions about Withdrawal or Withholding of Life Support. J Pediatr 190:118-123.e4 |
| Younge, Noelle; Goldstein, Ricki F; Bann, Carla M et al. (2017) Survival and Neurodevelopmental Outcomes among Periviable Infants. N Engl J Med 376:617-628 |
| Archer, Stephanie Wilson; Carlo, Waldemar A; Truog, William E et al. (2016) Improving publication rates in a collaborative clinical trials research network. Semin Perinatol 40:410-417 |
| Ahmed, Zuhayer; Prasad, Indrajit; Rahman, Hafizur et al. (2016) A Male with Extreme Subcutaneous Insulin Resistance: A Case Report. Rom J Diabetes Nutr Metab Dis 23:209-213 |
| Phelps, Dale L; Ward, Robert M; Williams, Rick L et al. (2016) Safety and pharmacokinetics of multiple dose myo-inositol in preterm infants. Pediatr Res 80:209-17 |
| Barroso, Julie; Leserman, Jane; Harmon, James L et al. (2015) Fatigue in HIV-Infected People: A Three-Year Observational Study. J Pain Symptom Manage 50:69-79 |
| Stafford-Smith, Mark; Li, Yi-Ju; Mathew, Joseph P et al. (2015) Genome-wide association study of acute kidney injury after coronary bypass graft surgery identifies susceptibility loci. Kidney Int 88:823-32 |
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