To study the novel bacterial-host cell interactions associated with intracellular infection by Legionella pneumophila , we developed a new transposon mutagenesis system. Mini-Tn10phoA generates translational gene fusions with the E. coli alkaline phosphatase gene, permitting us to identify insertions in genes encoding signal sequences. We generated a panel of 170 PhoA+ mutants and identified 23 that are unable to produce cytopathicity of U937 (macrophage-like) cells or to replicate in the amoebae, Hartmannella vermiformis. My hypothesis is that these avirulent mutants will have defects in intracellular trafficking, i.e., delivery to the lysosome or other non-permissive compartment. I am using immunofluorescence microscopy with antibodies directed at organelle-specific markers to determine the intracellular fate of these mutants. Ultimately, I expect to isolate bacterial factors that influence intracellular trafficking.

Project Start
1997-02-20
Project End
1997-11-30
Budget Start
1996-10-01
Budget End
1997-09-30
Support Year
37
Fiscal Year
1997
Total Cost
Indirect Cost
Name
University of Michigan Ann Arbor
Department
Type
DUNS #
791277940
City
Ann Arbor
State
MI
Country
United States
Zip Code
48109
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