This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This project serves as a human tissue core to provide tissues from metabolically characterized subjects with or without obesity and/or diabetes for several research aims including: 1. To describe incidence of impaired glucose tolerance and overt type 2 diabetes in morbidly obese subjects scheduled to undergo gastric bypass surgery, and subjects who have lost weight from bariatric surgery scheduled to undergo panniculectomy. 2. To assess gene regulation in insulin sensitive tissues of liver, muscle, intra-abdominal and extra-abdominal fat. 3. To correlate gene expression profiles to presence or absence of diabetes and degree of insulin sensitivity. 4. To evaluate proteins involved in appetite and weight regulation before and after weight loss. 5. To determine if cortisol metabolism differs in intraabdominal as compared to extra abdominal adipose compartments. 6. To determine the glucose tolerance and insulin sensitivity of subjects with non-alcoholic steatotic hepatitis (NASH), and evaluate gene profiling in liver tissue from subjects with and without NASH. 7. To evaluate expression levels of inflammatory proteins in hepatic and adipose tissues in lean, obese, and obese diabetic persons. 8. To determine whether selective insulin resistance exists in the microvessels of insulin resistant and type 2 diabetic patients and whether it can be correlated to PKC activation in monocytes and large vessel endothelial dysfunctions.
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