This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The purpose of the study is to determine the variability of measuring lipids in human skeletal muscle using proton magnetic resonance spectroscopy (1H-MRS). In vivo 1H-MRS of muscle can be performed with a high level of technical success, allowing identification of metabolically relevant biochemicals. Prior studies quantifying intramyocellular lipid (IMCL) using in vivo 1H-MRS have shown significant correlation with biochemical and electron microscopy measurements of muscle biopsy samples. Furthermore, several studies have demonstrated IMCL quantification with 1H-MRS is a reliable non-invasive surrogate marker of insulin sensitivity in healthy, obese and type 2 diabetic individuals. Although 1H-MRS represents a powerful technique for measuring intramyocellular lipids and other muscle metabolites, measurement repeatability must be established to confidently detect changes caused by disease, pharmacological or exercise protocols. Importantly, only a few studies have assessed the repeatability and variability of 1H-MRS IMCL quantification in healthy subjects. Technical factors, data analysis methodology, physical activity, diet, and medications may introduce variations of 1H-MRS IMCL quantification, potentially affecting its sensitivity to detect changes in longitudinal studies. Thus, the purpose of our study is to prospectively determine the repeatability and variability of intramyocellular lipid quantification using 1H-MR spectroscopy in healthy subjects at 1.5T and 3.0T magnetic field strengths.
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