This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The study is examining the genetic epidemiology of hypertension in a cohort of African Americans in the Washington, DC metropolitian area. The specific goals are to i) Enroll and examine a randomly acertained population-based cohort of 350 AA families with a minimum of 5 persons per family ii) Collect extensive demographic data (eg. adddress for anticipated follow-up, income education, smoking, drinking and household size) and family medical history on all participants , iii) Perform extensive phenotypic characterization, including: a) anthropometric and related variables: height, weight, waist and hip circumference, body composition variables, including fat mass (FM), fat free mass (FFM), and percent body fat (PBF) using Bioelectric Impedance Analyzer (BIA) in all participants b) BP and related physiologic intermediates including components of the renin-angiotensin aldosterone system (RAAS): angiotensinogen (AGT), angiotensin converting enzyme (ACE) and aldosterone. Measure other BP related intermediate pheotypes including endothelin 1 (ET1) and C-reactive protein(CRP), c) diabetes related variables: glucose, c-peptide and insulin in all participants. iv) Evaluate the association and linkage between BP, HTN and genes of the RAAS (ACE, AGT and angiotensin II receptor type 1- AT2R1) and other selected condidate genes including epithelial sodium channel beta subunit (ENaC), endothelial nitic oxide synthases (eNOSs, CRP, ET1, and beta2-adrenergic receptor (ADRB2). Probands satisfying the age requirement (i.e., 30-60 years) and family structure criteria are identified from randomly selected, predominantly African American communities from the DC metropolitan area, after which the proband and consenting family members are scheduled for a clinic appointment in the GCRC. Participants are requested to fast for a minimum of eight hours on the night preceding the clinic appointment. At the GCRC, written informed consent is obtained following which demographic data and a medical history are obtained. Anthropometric, blood pressure and body composition measurements are done using standardized methods. Fasting blood samples are obtained from each participant comprising two 10ml EDTA tubes for plasma and buffy coat (source of DNA), one 15ml red top for serum and a 5 ml gray top for fasting glucose measurement. Spot urine are also obtained from all participants.
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