Free Radicals &Age-Induced Neurochemical Changes in the Monkey

Volicer, Ladislav

Abstract

The goal of this investigation is to determine the role of oxygen-derived free radicals in aging of the brain. This goal will be approached by obtaining measurements of the effect of age on different aspects of free formation and metabolism in several areas of monkey brain. The parameters to be measured include lipid peroxidation, activity of superoxide dismutase, glutathione peroxidation, and catalase, concentrations of free radical scavengers including glutathione, ascorbic acid and uric acid, and the ability of brain supernatant to inhibit free radical formation. Our method of scavenger measurement, HPLC with coulometric detection using a 16 channel detector system, will allow us to determine simultaneously concentrations of monoamines, their precursors and metabolites in the same tissue sample. Additional information on the activity of selected neurotransmitter systems will be obtained by the measurement of choline acetyltransferase activity and GABA concentrations. Relationship between free radical metabolism and neurotransmitter levels will be explored. In addition, all variables measured in this project will be correlated with behavioral, biochemical and morphological age-related changes found in the same monkeys.

Funding Agency

• Agency: National Institute of Health (NIH)
• Institute: National Institute on Aging (NIA)
• Type: Research Program Projects (P01)
• Project #: 5P01AG000001-20
• Application #: 3745417
• Support Year: 20
• Fiscal Year: 1994

Institution

• Name: Boston University
• DUNS #: 604483045
• City: Boston
• State: MA
• Country: United States
• Zip Code: 02118

Publications

Robinson, Amy A; Abraham, Carmela R; Rosene, Douglas L (2018) Candidate molecular pathways of white matter vulnerability in the brain of normal aging rhesus monkeys. Geroscience 40:31-47

Ragan, I K; Davis, A S; McVey, D S et al. (2018) Evaluation of Fluorescence Microsphere Immunoassay for Detection of Antibodies to Rift Valley Fever Virus Nucleocapsid Protein and Glycoproteins. J Clin Microbiol 56:

Moore, Tara L; Bowley, Bethany G E; Shultz, Penny L et al. (2018) Oral curcumin supplementation improves fine motor function in the middle-aged rhesus monkey. Somatosens Mot Res 35:1-10

Hsu, Alexander; Luebke, Jennifer I; Medalla, Maria (2017) Comparative ultrastructural features of excitatory synapses in the visual and frontal cortices of the adult mouse and monkey. J Comp Neurol 525:2175-2191

Shobin, Eli; Bowley, Michael P; Estrada, Larissa I et al. (2017) Microglia activation and phagocytosis: relationship with aging and cognitive impairment in the rhesus monkey. Geroscience 39:199-220

Estrada, Larissa I; Robinson, Amy A; Amaral, Ana C et al. (2017) Evaluation of Long-Term Cryostorage of Brain Tissue Sections for Quantitative Histochemistry. J Histochem Cytochem 65:153-171

Medalla, Maria; Gilman, Joshua P; Wang, Jing-Yi et al. (2017) Strength and Diversity of Inhibitory Signaling Differentiates Primate Anterior Cingulate from Lateral Prefrontal Cortex. J Neurosci 37:4717-4734

Rumbell, Timothy H; Dragulji?, Danel; Yadav, Aniruddha et al. (2016) Automated evolutionary optimization of ion channel conductances and kinetics in models of young and aged rhesus monkey pyramidal neurons. J Comput Neurosci 41:65-90

Wilson, William C; Davis, A Sally; Gaudreault, Natasha N et al. (2016) Experimental Infection of Calves by Two Genetically-Distinct Strains of Rift Valley Fever Virus. Viruses 8:

Shivanna, Vinay; McDowell, Chester; Wilson, William C et al. (2016) Complete Genome Sequence of Two Rift Valley Fever Virus Strains Isolated from Outbreaks in Saudi Arabia (2000) and Kenya (2006 to 2007). Genome Announc 4: