Abstract

Our projects cover a multidisciplinary, but coherent approach directed toward an understanding of unconventional viral dementias and their relationship to """"""""non infectious"""""""" dementias (e.g. Alzheimer's disease). Experimental CJD rodent models will be used to evaluate critical events during infection and progression of disease. Biochemical and genetic studies in parallel with morphological and infectivity studies are here proposed. We continue our search for true agent-specific (non-host) molecules (protein and nucleic acid). We also hope to define probes that will be of use in studies of Alzheimer's disease as well as in CJD. Such probes will facilitate comparisons of infectious dementias with those of unknown etiology, and will be used to analyze both experimental rodent material and precious human specimen of CJD, GSS and AD.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute on Aging (NIA)
Type
Research Program Projects (P01)
Project #
5P01AG003106-08
Application #
3090660
Study Section
Aging Review Committee (AGE)
Project Start
1982-09-30
Project End
1992-11-30
Budget Start
1989-12-01
Budget End
1990-11-30
Support Year
8
Fiscal Year
1990
Total Cost
Indirect Cost

  Institution

Name
Yale University
Department
Type
Schools of Medicine
DUNS #
082359691
City
New Haven
State
CT
Country
United States
Zip Code
06520

  Publications

DeGiorgio, Lorraine A; Manuelidis, Laura; Bernstein, Jerald J (2002) Transient appearance of amyloid precursor protein plaques in the brain of thymectomized rats after human leptomeningeal cell grafts. Neurosci Lett 322:62-6
Behar, K L; Boucher, R; Fritch, W et al. (1998) Changes in N-acetylaspartate and myo-inositol detected in the cerebral cortex of hamsters with Creutzfeldt-Jakob disease. Magn Reson Imaging 16:963-8
Manuelidis, L (1994) The dimensions of Creutzfeldt-Jakob disease. Transfusion 34:915-28
Sklaviadis, T; Dreyer, R; Manuelidis, L (1992) Analysis of Creutzfeldt-Jakob disease infectious fractions by gel permeation chromatography and sedimentation field flow fractionation. Virus Res 26:241-54
Taruscio, D; Manuelidis, L (1991) Integration site preferences of endogenous retroviruses. Chromosoma 101:141-56
Manuelidis, E E; Manuelidis, L (1991) Search for a transmissible agent in Alzheimer's disease: studies of human buffy coat. Curr Top Microbiol Immunol 172:275-80
Akowitz, A; Sklaviadis, T; Manuelidis, E E et al. (1990) Nuclease-resistant polyadenylated RNAs of significant size are detected by PCR in highly purified Creutzfeldt-Jakob disease preparations. Microb Pathog 9:33-45
Murdoch, G H; Sklaviadis, T; Manuelidis, E E et al. (1990) Potential retroviral RNAs in Creutzfeldt-Jakob disease. J Virol 64:1477-86
Sklaviadis, T; Akowitz, A; Manuelidis, E E et al. (1990) Nuclease treatment results in high specific purification of Creutzfeldt-Jakob disease infectivity with a density characteristic of nucleic acid-protein complexes. Arch Virol 112:215-28
Sklaviadis, T K; Manuelidis, L; Manuelidis, E E (1989) Physical properties of the Creutzfeldt-Jakob disease agent. J Virol 63:1212-22

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