Abstract

This program continues investigations on several aspects of the biology of aging cells and tissues. Certain aspects of biochemical and biomolecular processes which are age-dependent are being examined and include: a) vitamin K-dependent protein carboxylation and the synthesis of the bone protein, osteocalcin; b) osteocalcin and its relationships to the lipid-soluble vitamins in osteoporosis and other bone disorders; c) polyamine-dependent lysyl residue aminobutylation and hydroxylation and the synthesis of an 18,000 dalton hypusine-containing protein, which is a measure of cell proliferative capacity; d) lipoprotein metabolism and the aging process and the continued characterization of genes which code for certain apolipoproteins; e) the design and use of certain vital reagents which are based on special chemical features found in organoboron and aromatic quaterary compounds and use fluorescence and boron-NMR techniques.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute on Aging (NIA)
Type
Research Program Projects (P01)
Project #
5P01AG004727-06
Application #
3090912
Study Section
Aging Review Committee (AGE)
Project Start
1978-05-01
Project End
1988-04-30
Budget Start
1987-05-01
Budget End
1988-04-30
Support Year
6
Fiscal Year
1987
Total Cost
Indirect Cost

  Institution

Name
Children's Hospital Boston
Department
Type
DUNS #
076593722
City
Boston
State
MA
Country
United States
Zip Code
02115

  Publications

al-Rayes, H; Pachas, W; Mirza, N et al. (1992) IgE regulation and lymphokine patterns in aging humans. J Allergy Clin Immunol 90:630-6
Paz, M A; Fluckiger, R; Boak, A et al. (1991) Specific detection of quinoproteins by redox-cycling staining. J Biol Chem 266:689-92
Vercelli, D; Jabara, H H; Cunningham-Rundles, C et al. (1990) Regulation of immunoglobulin (Ig)E synthesis in the hyper-IgE syndrome. J Clin Invest 85:1666-71
Jabara, H H; Fu, S M; Geha, R S et al. (1990) CD40 and IgE: synergism between anti-CD40 monoclonal antibody and interleukin 4 in the induction of IgE synthesis by highly purified human B cells. J Exp Med 172:1861-4
Jabara, H H; Schneider, L C; Shapira, S K et al. (1990) Induction of germ-line and mature C epsilon transcripts in human B cells stimulated with rIL-4 and EBV. J Immunol 145:3468-73
Vercelli, D; Jabara, H H; Lauener, R P et al. (1990) IL-4 inhibits the synthesis of IFN-gamma and induces the synthesis of IgE in human mixed lymphocyte cultures. J Immunol 144:570-3
Veldink, G A; Boelens, H; Maccarrone, M et al. (1990) Soybean lipoxygenase-1 is not a quinoprotein. FEBS Lett 270:135-8
Gallop, P M; Henson, E; Paz, M A et al. (1989) Acid-promoted tautomeric lactonization and oxidation-reduction of pyrroloquinoline quinone (PQQ). Biochem Biophys Res Commun 163:755-63
Strang, C J; Henson, E; Okamoto, Y et al. (1989) Separation and determination of alpha-amino acids by boroxazolidone formation. Anal Biochem 178:276-86
Weintraub, M S; Rosen, Y; Otto, R et al. (1989) Physical exercise conditioning in the absence of weight loss reduces fasting and postprandial triglyceride-rich lipoprotein levels. Circulation 79:1007-14

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