This project involves the development and validation of radio-immunoassays for the measurement of """"""""bone-specific"""""""" proteins in serum and urine. These will be used to assess bone formation and resorption in age-related bone loss and metabolic bone diseases. At first, the focus will be on bone Gla protein, osteonectin; then, on other noncollagenous bone-specific proteins, as these are isolated in Project 0003 and antibodies are developed. Assay of skeletal alkaline phosphatase which is near clinical usefulness will also be utilized in this project. A library of monoclonal and polyclonal antibodies to the proteins isolated on Project 0003 will be developed. Normal values will be established in healthy individuals of both sexes from ages 20-90 years. Criteria for exclusion of patients with bone disease are outlined. Studies will then be performed in patients with osteoporosis, hyperparathyroidism, hyperthyroidism, Paget's disease, steriod-induced osteoporosis, and other metabolic bone diseases, as well as patients with osteogenic sarcoma, and carcinoma of the prostate. The relationship to vitamin D metabolism will be evaluated by comparing serum levels in patients with vitamin D deficiency and subjects with hypervitaminosis D.

Agency
National Institute of Health (NIH)
Institute
National Institute on Aging (NIA)
Type
Research Program Projects (P01)
Project #
5P01AG004875-04
Application #
3821723
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
4
Fiscal Year
1987
Total Cost
Indirect Cost
Name
Mayo Clinic, Rochester
Department
Type
DUNS #
City
Rochester
State
MN
Country
United States
Zip Code
55905
Xu, Ming; Pirtskhalava, Tamar; Farr, Joshua N et al. (2018) Senolytics improve physical function and increase lifespan in old age. Nat Med 24:1246-1256
Khosla, Sundeep; Farr, Joshua N; Kirkland, James L (2018) Inhibiting Cellular Senescence: A New Therapeutic Paradigm for Age-Related Osteoporosis. J Clin Endocrinol Metab 103:1282-1290
Rocca, Walter A (2018) The future burden of Parkinson's disease. Mov Disord 33:8-9
Rocca, Walter A; Gazzuola Rocca, Liliana; Smith, Carin Y et al. (2018) Personal, reproductive, and familial characteristics associated with bilateral oophorectomy in premenopausal women: A population-based case-control study. Maturitas 117:64-77
Drake, Matthew T; Fenske, Jennifer S; Blocki, Frank A et al. (2018) Validation of a novel, rapid, high precision sclerostin assay not confounded by sclerostin fragments. Bone 111:36-43
Laughlin-Tommaso, Shannon K; Khan, Zaraq; Weaver, Amy L et al. (2018) Cardiovascular and metabolic morbidity after hysterectomy with ovarian conservation: a cohort study. Menopause 25:483-492
Farr, Joshua N; Weivoda, Megan M; Nicks, Kristy M et al. (2018) Osteoprotection Through the Deletion of the Transcription Factor Ror? in Mice. J Bone Miner Res 33:720-731
Wenning, Gregor; Trojanowski, John Q; Kaufmann, Horacio et al. (2018) Is multiple system atrophy an infectious disease? Ann Neurol 83:10-12
Kattah, Andrea G; Smith, Carin Y; Gazzuola Rocca, Liliana et al. (2018) CKD in Patients with Bilateral Oophorectomy. Clin J Am Soc Nephrol 13:1649-1658
Khosla, Sundeep; Monroe, David G (2018) Regulation of Bone Metabolism by Sex Steroids. Cold Spring Harb Perspect Med 8:

Showing the most recent 10 out of 401 publications