This project involves the development and validation of radio-immunoassays for the measurement of """"""""bone-specific"""""""" proteins in serum and urine. These will be used to assess bone formation and resorption in age-related bone loss and metabolic bone diseases. At first, the focus will be on bone Gla protein, osteonectin; then, on other noncollagenous bone-specific proteins, as these are isolated in Project 0003 and antibodies are developed. Assay of skeletal alkaline phosphatase which is near clinical usefulness will also be utilized in this project. A library of monoclonal and polyclonal antibodies to the proteins isolated on Project 0003 will be developed. Normal values will be established in healthy individuals of both sexes from ages 20-90 years. Criteria for exclusion of patients with bone disease are outlined. Studies will then be performed in patients with osteoporosis, hyperparathyroidism, hyperthyroidism, Paget's disease, steriod-induced osteoporosis, and other metabolic bone diseases, as well as patients with osteogenic sarcoma, and carcinoma of the prostate. The relationship to vitamin D metabolism will be evaluated by comparing serum levels in patients with vitamin D deficiency and subjects with hypervitaminosis D.
| Kattah, Andrea G; Smith, Carin Y; Gazzuola Rocca, Liliana et al. (2018) CKD in Patients with Bilateral Oophorectomy. Clin J Am Soc Nephrol 13:1649-1658 |
| Khosla, Sundeep; Monroe, David G (2018) Regulation of Bone Metabolism by Sex Steroids. Cold Spring Harb Perspect Med 8: |
| Bower, James H; Grossardt, Brandon R; Rocca, Walter A et al. (2018) Prevalence of and indications for antipsychotic use in Parkinson's disease. Mov Disord 33:325-328 |
| Rocca, Walter A; Grossardt, Brandon R; Brue, Scott M et al. (2018) Data Resource Profile: Expansion of the Rochester Epidemiology Project medical records-linkage system (E-REP). Int J Epidemiol 47:368-368j |
| Xu, Ming; Pirtskhalava, Tamar; Farr, Joshua N et al. (2018) Senolytics improve physical function and increase lifespan in old age. Nat Med 24:1246-1256 |
| Khosla, Sundeep; Farr, Joshua N; Kirkland, James L (2018) Inhibiting Cellular Senescence: A New Therapeutic Paradigm for Age-Related Osteoporosis. J Clin Endocrinol Metab 103:1282-1290 |
| Rocca, Walter A (2018) The future burden of Parkinson's disease. Mov Disord 33:8-9 |
| Rocca, Walter A; Gazzuola Rocca, Liliana; Smith, Carin Y et al. (2018) Personal, reproductive, and familial characteristics associated with bilateral oophorectomy in premenopausal women: A population-based case-control study. Maturitas 117:64-77 |
| Drake, Matthew T; Fenske, Jennifer S; Blocki, Frank A et al. (2018) Validation of a novel, rapid, high precision sclerostin assay not confounded by sclerostin fragments. Bone 111:36-43 |
| Laughlin-Tommaso, Shannon K; Khan, Zaraq; Weaver, Amy L et al. (2018) Cardiovascular and metabolic morbidity after hysterectomy with ovarian conservation: a cohort study. Menopause 25:483-492 |
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