This program project will primarily use genetic modified mice. The Animal Core will provide essentialgenetically modified mice and control wild-type mice to support the research of all four projects. Toaccomplish this objective, the Animal Core will be responsible for: 1) establishing, maintaining and breedingthe required numbers and ages of knock-in, knockout and transgenic mice; 2) characterizing gene alteredmice using molecular, biochemical, and pathological methods to ensure genotype and phenotypes; 3)performing the complex genetic crosses required to obtain mice with the desired combinations of mutations;and 4) providing pups at the desired gestation stages and ages to establish primary culture cells. Bycoordinating and standardizing animal and cell use among projects, animals, money, and time will be saved.Providing every project with animals and cells derived in the same manner will make it possible to crosscorrelateinformation from the projects, which will result in synergistic use of the animals and the datagenerated. The Animal Core will also work closely with the Administrative Core and Core B to help theproject leaders plan and/or define their studies.
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