Abstract

The tissue core will process human articular cartilage and isolate chondrocytes. Cells will be stimulated in high density primary culture or in alginate beads and provided to the projects for experiments. Protein and mRNA extracts will be prepared from these specimens. The studies proposed in all research components depend on these samples and this core is thus essential to the success of the program. The specific functions of the core are: 1) Isolation of chondrocytes and meniscus cells. 2) Isolation of RNA from each donor knee. 3) Maintenance of a cell bank with chondrocytes from different age groups. 4) Chondrocyte cultures in monolayer and alginate. 5) Preparation of cells, conditioned media, protein and nucleic acid extracts from different culture systems and stimulation conditions. 6) Biomechanical stimulation of tissue explants.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute on Aging (NIA)
Type
Research Program Projects (P01)
Project #
2P01AG007996-12
Application #
6481521
Study Section
Special Emphasis Panel (ZAG1-ZIJ-2 (J2))
Project Start
1997-07-01
Project End
2007-03-31
Budget Start
Budget End
Support Year
12
Fiscal Year
2002
Total Cost
$267,700
Indirect Cost

  Institution

Name
Scripps Research Institute
Department
Type
DUNS #
City
La Jolla
State
CA
Country
United States
Zip Code
92037

  Publications

Jin, Yunyun; Cong, Qian; Gvozdenovic-Jeremic, Jelena et al. (2018) Enpp1 inhibits ectopic joint calcification and maintains articular chondrocytes by repressing hedgehog signaling. Development 145:
Grogan, Shawn P; Duffy, Stuart F; Pauli, Chantal et al. (2018) Gene expression profiles of the meniscus avascular phenotype: A guide for meniscus tissue engineering. J Orthop Res 36:1947-1958
Baek, Jihye; Sovani, Sujata; Choi, Wonchul et al. (2018) Meniscal Tissue Engineering Using Aligned Collagen Fibrous Scaffolds: Comparison of Different Human Cell Sources. Tissue Eng Part A 24:81-93
Chen, L-Y; Wang, Y; Terkeltaub, R et al. (2018) Activation of AMPK-SIRT3 signaling is chondroprotective by preserving mitochondrial DNA integrity and function. Osteoarthritis Cartilage 26:1539-1550
Shadyab, A H; Terkeltaub, R; Kooperberg, C et al. (2018) Prospective associations of C-reactive protein (CRP) levels and CRP genetic risk scores with risk of total knee and hip replacement for osteoarthritis in a diverse cohort. Osteoarthritis Cartilage 26:1038-1044
Ishitobi, Hiroyuki; Sanada, Yohei; Kato, Yoshio et al. (2018) Carnosic acid attenuates cartilage degeneration through induction of heme oxygenase-1 in human articular chondrocytes. Eur J Pharmacol 830:1-8
Alvarez-Garcia, Oscar; Matsuzaki, Tokio; Olmer, Merissa et al. (2018) FOXO are required for intervertebral disk homeostasis during aging and their deficiency promotes disk degeneration. Aging Cell 17:e12800
Miyaki, Shigeru; Lotz, Martin K (2018) Extracellular vesicles in cartilage homeostasis and osteoarthritis. Curr Opin Rheumatol 30:129-135
Kalyanaraman, Hema; Schwaerzer, Gerburg; Ramdani, Ghania et al. (2018) Protein Kinase G Activation Reverses Oxidative Stress and Restores Osteoblast Function and Bone Formation in Male Mice With Type 1 Diabetes. Diabetes 67:607-623
Lee, Kwang Il; Olmer, Merissa; Baek, Jihye et al. (2018) Platelet-derived growth factor-coated decellularized meniscus scaffold for integrative healing of meniscus tears. Acta Biomater 76:126-134

Showing the most recent 10 out of 321 publications