Abstract

The molecular mechanism involved in neuronal cell death associated with aging have not been fully explained. This project proposes to examine important sites which have been implicated in cell death: the voltage- dependent calcium channels of neuronal membranes and the intracellular calcium release channels in the endoplasmic reticulum. An understanding of the structure, function, and regulation of these critical sites in normal, aging models, and aged animals could provide the necessary background for successful therapeutic intervention. A second aspect of this project will be to develop antibodies, both monoclonal and polyclonal, which will allow the localization and visualization of calcium channels and important receptors. Chemical probes for calcium channels and for sites involved in IP3 action will also be developed. The antibodies and chemical probes will be a resource for other investigations in our overall program and will be invaluable to the scientific community in general.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute on Aging (NIA)
Type
Research Program Projects (P01)
Project #
5P01AG009480-04
Application #
3746078
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
4
Fiscal Year
1994
Total Cost
Indirect Cost

  Institution

Name
New York University
Department
Type
DUNS #
004514360
City
New York
State
NY
Country
United States
Zip Code
10012

  Publications

Kandiel, A; Chen, S; Hillman, D E (1999) c-fos gene expression parallels auditory adaptation in the adult rat. Brain Res 839:292-7
Chen, S; Hillman, D E (1999) Dying-back of Purkinje cell dendrites with synapse loss in aging rats. J Neurocytol 28:187-96
Chen, S; Ren, Y Q; Bing, G Y et al. (1998) Transient c-fos gene expression in cerebellar development and functional stimulation. Brain Res 795:87-97
Apicella, S; Chen, S; Bing, R et al. (1997) Plasmalemmal ATPase calcium pump localizes to inner and outer hair bundles. Neuroscience 79:1145-51
Hillman, D E; Gordon, C E; Troublefield, Y et al. (1997) Effect of unilateral tympanotomy on auditory induced c-fos expression in cochlear nuclei. Brain Res 748:77-84
Chen, S; Ren, Y Q; Hillman, D E (1996) Transient expression of lyn gene in Purkinje cells during cerebellar development. Brain Res Dev Brain Res 92:140-6
Hillman, D E; Chen, S; Bing, R et al. (1996) Ultrastructural localization of the plasmalemmal calcium pump in cerebellar neurons. Neuroscience 72:315-24
Cherksey, B D; Sapirstein, V S; Geraci, A L (1996) Adrenal chromaffin cells on microcarriers exhibit enhanced long-term functional effects when implanted into the mammalian brain. Neuroscience 75:657-64
Chen, S; Bing, R; Rosenblum, N et al. (1996) Immunohistochemical localization of Lyn (p56) protein in the adult rat brain. Neuroscience 71:89-100
Denk, W; Sugimori, M; Llinas, R (1995) Two types of calcium response limited to single spines in cerebellar Purkinje cells. Proc Natl Acad Sci U S A 92:8279-82

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