Abstract

The Tissue Culture Core Facility will serve to aid the Project's investigators by the growth and storage of cell lines, the purification of monoclonal antibody biotinylation of proteins, and magnetic fell sorting. Further, training and assistance in histologic methodologies including in situ hybridization will be provided. By serving as central repository for cell lines and antibodies and providing proven techniques to the Program Project, the Core will allow investigators to increase the scientific output of their laboratories.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute on Aging (NIA)
Type
Research Program Projects (P01)
Project #
5P01AG010207-02
Application #
3768504
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
2
Fiscal Year
1993
Total Cost
Indirect Cost

  Institution

Name
University of Maryland Baltimore
Department
Type
DUNS #
003255213
City
Baltimore
State
MD
Country
United States
Zip Code
21201

  Publications

Han, Shuhua; Yang, Kaiyong; Ozen, Zeynep et al. (2003) Enhanced differentiation of splenic plasma cells but diminished long-lived high-affinity bone marrow plasma cells in aged mice. J Immunol 170:1267-73
Dal Porto, Joseph M; Haberman, Ann M; Kelsoe, Garnett et al. (2002) Very low affinity B cells form germinal centers, become memory B cells, and participate in secondary immune responses when higher affinity competition is reduced. J Exp Med 195:1215-21
Davila, M; Foster, S; Kelsoe, G et al. (2001) A role for secondary V(D)J recombination in oncogenic chromosomal translocations? Adv Cancer Res 81:61-92
Lu, Y F; Singh, M; Cerny, J (2001) Canonical germinal center B cells may not dominate the memory response to antigenic challenge. Int Immunol 13:643-55
Chen, Z; Koralov, S B; Kelsoe, G (2000) Regulation of humoral immune responses by CD21/CD35. Immunol Rev 176:194-204
Chen, Z; Koralov, S B; Kelsoe, G (2000) Complement C4 inhibits systemic autoimmunity through a mechanism independent of complement receptors CR1 and CR2. J Exp Med 192:1339-52
Takahashi, Y; Cerasoli, D M; Dal Porto, J M et al. (1999) Relaxed negative selection in germinal centers and impaired affinity maturation in bcl-xL transgenic mice. J Exp Med 190:399-410
Yang, K; Davila, M; Kelsoe, G (1999) Do germinal centers have a role in the generation of lymphomas? Curr Top Microbiol Immunol 246:53-60;discussion 61-2
Song, H; Nie, X; Basu, S et al. (1999) Regulation of VH gene repertoire and somatic mutation in germinal centre B cells by passively administered antibody. Immunology 98:258-66
Dal Porto, J M; Haberman, A M; Shlomchik, M J et al. (1998) Antigen drives very low affinity B cells to become plasmacytes and enter germinal centers. J Immunol 161:5373-81

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