The purpose of the Neuroimaging Core (Core C) is to provide access to equipment and technical expertise for confocai microscopy, epi-fluorescence microscopy, and image processing and analysis. The range of experiments that will take advantage of this core include immunofluorescence, imaging of GFP and other organelle- and protein-specific dyes, time-lapse confocal microscopy, calcium imaging, and three-dimensional reconstruction and deconvolution of cells and tissue relevant to identify properties of intracellular calcium signaling mechanisms. Core C will take advantage of the University of North Texas Health Science Center's existing equipment in order to provide these services. Equipment in the core will include a digital imaging workstation with fluorescence microscope (Olympus IX70), fast CCD camera (ORCA ER C4742) and excitation wavelength switcher that provides wavelengths for dye excitation and uncaging (Sutter DG-4), a luminescence spectrofluorometer (AMINCO-Bowman Series 2), a Zeiss LSM 410 confocal microscope, a Bio-Rad Radiance 2100 confocal microscope and two computer workstations for off-line image processing and data analysis. Core C will also provide a Research Support Specialist who is experienced in fluorescence, deconvolution, confocal and multi-photon microscopy. This individual will assist members of each project with all experiments requiring the use of this core facility.

Agency
National Institute of Health (NIH)
Institute
National Institute on Aging (NIA)
Type
Research Program Projects (P01)
Project #
5P01AG010485-17
Application #
7569464
Study Section
Special Emphasis Panel (ZAG1)
Project Start
Project End
Budget Start
2008-02-01
Budget End
2009-01-31
Support Year
17
Fiscal Year
2008
Total Cost
$89,396
Indirect Cost
Name
University of North Texas
Department
Type
DUNS #
110091808
City
Fort Worth
State
TX
Country
United States
Zip Code
76107
Richter, Frank; Koulen, Peter; Kaja, Simon (2016) N-Palmitoylethanolamine Prevents the Run-down of Amplitudes in Cortical Spreading Depression Possibly Implicating Proinflammatory Cytokine Release. Sci Rep 6:23481
Means, John C; Gerdes, Bryan C; Kaja, Simon et al. (2016) Caspase-3-Dependent Proteolytic Cleavage of Tau Causes Neurofibrillary Tangles and Results in Cognitive Impairment During Normal Aging. Neurochem Res 41:2278-88
Montgomery, Christa L; Keereetaweep, Jantana; Johnson, Heather M et al. (2016) Changes in Retinal N-Acylethanolamines and their Oxylipin Derivatives During the Development of Visual Impairment in a Mouse Model for Glaucoma. Lipids 51:857-66
Kaja, Simon; Payne, Andrew J; Singh, Tulsi et al. (2015) An optimized lactate dehydrogenase release assay for screening of drug candidates in neuroscience. J Pharmacol Toxicol Methods 73:1-6
Sarkar, Saumyendra; Jun, Sujung; Simpkins, James W (2015) Estrogen amelioration of A?-induced defects in mitochondria is mediated by mitochondrial signaling pathway involving ER?, AKAP and Drp1. Brain Res 1616:101-11
Kaja, Simon; Payne, Andrew J; Naumchuk, Yuliya et al. (2015) Plate reader-based cell viability assays for glioprotection using primary rat optic nerve head astrocytes. Exp Eye Res 138:159-66
Cheli, V T; Santiago González, D A; Spreuer, V et al. (2015) Voltage-gated Ca2+ entry promotes oligodendrocyte progenitor cell maturation and myelination in vitro. Exp Neurol 265:69-83
Kaja, Simon; Sumien, Nathalie; Shah, Vidhi V et al. (2015) Loss of Spatial Memory, Learning, and Motor Function During Normal Aging Is Accompanied by Changes in Brain Presenilin 1 and 2 Expression Levels. Mol Neurobiol 52:545-54
Kaja, S; Payne, A J; Nielsen, E Ø et al. (2015) Differential cerebellar GABAA receptor expression in mice with mutations in CaV2.1 (P/Q-type) calcium channels. Neuroscience 304:198-208
Grillo, Stephanie L; Koulen, Peter (2015) Psychophysical testing in rodent models of glaucomatous optic neuropathy. Exp Eye Res 141:154-63

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