Abstract

Fetal bovine serum is used by all projects and will be tested for optimal growth and cloning efficiency by Core. All the projects will utilize the lot of fetal bovine serum characterized as the best for use, and this will allow for purchase of large amounts of a particular lot for a better price. The Core will also perform regular mycoplasma testing on the various cell cultures used in the individual projects. In addition, Projects 1, 2, 3 and 5 will make use of the microinjection/electroporation facility to introduce macromolecules (primarily RNA, DNA and protein) into cultured mammalian cells. This centralized facility will provide equipment, supplies, and personnel for the whole program. This is a very cost efficient way for all of the investigators to have access to techniques requiring skilled personnel and specialized equipment.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute on Aging (NIA)
Type
Research Program Projects (P01)
Project #
5P01AG013663-03
Application #
6267678
Study Section
Project Start
1998-04-01
Project End
1999-03-31
Budget Start
1997-10-01
Budget End
1998-09-30
Support Year
3
Fiscal Year
1998
Total Cost
Indirect Cost

  Institution

Name
Baylor College of Medicine
Department
Type
DUNS #
074615394
City
Houston
State
TX
Country
United States
Zip Code
77030

  Publications

Young, Juan I; Sedivy, John M; Smith, James R (2003) Telomerase expression in normal human fibroblasts stabilizes DNA 5-methylcytosine transferase I. J Biol Chem 278:19904-8
Thomas, Michael; Suwa, Tetsuya; Yang, Lianqing et al. (2002) Cooperation of hTERT, SV40 T antigen and oncogenic Ras in tumorigenesis: a cell transplantation model using bovine adrenocortical cells. Neoplasia 4:493-500
Zhang, Hao; Hornsby, Peter J (2002) Intradermal cell transplantation in soluble collagen. Cell Transplant 11:139-45
Hornsby, Peter J (2002) Aging of the human adrenal cortex. Ageing Res Rev 1:229-42
Tominaga, Kaoru; Pereira-Smith, Olivia M (2002) The genomic organization, promoter position and expression profile of the mouse MRG15 gene. Gene 294:215-24
Pardo, Patricia S; Leung, James K; Lucchesi, John C et al. (2002) MRG15, a novel chromodomain protein, is present in two distinct multiprotein complexes involved in transcriptional activation. J Biol Chem 277:50860-6
Hornsby, Peter J; Yang, Lianqing; Thomas, Michael (2002) Adrenocortical cell proliferation in a cell transplantation model: the role of SV40 T antigen. Endocr Res 28:777-83
Hornsby, Peter J (2002) Cellular senescence and tissue aging in vivo. J Gerontol A Biol Sci Med Sci 57:B251-6
Thomas, Michael; Wang, Xiangdong; Hornsby, Peter J (2002) Human adrenocortical cell xenotransplantation: model of cotransplantation of human adrenocortical cells and 3T3 cells in scid mice to form vascularized functional tissue and prevent adrenal insufficiency. Xenotransplantation 9:58-67
Tominaga, Kaoru; Olgun, Abdullah; Smith, James R et al. (2002) Genetics of cellular senescence. Mech Ageing Dev 123:927-36

Showing the most recent 10 out of 47 publications