The goal of the Animal Management and Transgenic Mouse Core is to provide transgenic and the indicatedknockout animals bred into distinct genetic backgrounds to each of the three projects of this ProgramProject. Due to the extensive numbers of mice that are required for each project, and since some of thegenotypes are shared across more than one project, in order to ensure optimal quality control, mouseidentification and smooth flow of animals, the functions of breeding and genotyping of animals are best donewithin a core by a team dedicated to that function. Further, the ordering, receipt, entry into the database,and distribution of aged and young Fischer 344 from the NIA colonies will be managed by Core C. In CoreC, this team will be led by Dr. Shi Fang Van, and co-lead by Dr. Thomas Martin of the Institute ofComparative Medicine. Dr. Van has extensive experience in the breeding and genotyping of transgenicmice. Mechanisms are in place in Core C to facilitate mouse breeding, weaning, tattooing, tail biopsy andSouthern blotting/PCR to determine genotype. Multiple quality controls are in place to ensure consistency ofthe strains by Southern, Northern, Western blotting of representative mice per strain at specified intervals,along with activity assays as indicated. Dr. Martin has extensive experience in the care and use of mice andhas designed a thorough description of the care and management of aged mouse in our colonies. Core Cwill serve each of the three projects during each of the five years of the Program Project.
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