The Tissue and Neurobehavioral Phenotyping Core, designated as Core C, is a centralized facility that will provide investigators with a well-established and reproducible model of influenza A?induced pneumonia. Quantitative tools such as Flexivent measurements of lung mechanics, magnetic resonance imaging to evaluate lung repair and remodeling, and multiplex measures of pro-inflammatory cytokines will be conducted by Core C according to the research plan outlined in Projects 1, 2, and 3. Core C will use advanced flow cytometry capabilities to quantify, phenotype, and sort specific inflammatory and parenchymal cell populations from the murine lung, brain, and muscle. The sorting and isolation capabilities of the Core will allow cell-type- specific assessment of proteostasis networks via mass spectroscopy (as outlined by Core B). Core C will breed mice to generate the cell-type- or tissue-specific Cre recombinase lines to induce tissue-specific knockout mice. Core C will perform the genotyping of all the murine strains proposed by Project Leaders. Core C will maintain a uniform environment in which wild-type and genetically engineered mice will be aged. Importantly, Core C will assess the impact of influenza A?induced pneumonia on age-related changes in neurobehavioral phenotypes. Briefly, Core C will examine motor coordination and balance by means of an accelerating rotarod test. Response latency on a hot plate will be tested to assess nociceptive functions. Exploratory behaviors and locomotor activity will be examined in an open field assay and by continuous monitoring with running wheels. Learning and memory will be examined in novel object recognition and Barnes maze tests. Collectively, these tools provide a unique resource for Project Investigators, which would be difficult to reproduce without the support of this PPG.
It is not required per instructions stated on the Funding Opportunity Announcement PAR-18-297, Section IV. Application and Submission Information, Project, Research & Related Other Project Information (Core), ?Project Narrative: Do not complete?.
| Sala, Marc A; Balderas-Martínez, Yalbi Itzel; Buendía-Roldan, Ivette et al. (2018) Inflammatory pathways are upregulated in the nasal epithelium in patients with idiopathic pulmonary fibrosis. Respir Res 19:233 |
| Dela Cruz, Charles S; Wunderink, Richard G; Christiani, David C et al. (2018) Future Research Directions in Pneumonia. NHLBI Working Group Report. Am J Respir Crit Care Med 198:256-263 |
| Galluzzi, Lorenzo; Vitale, Ilio; Aaronson, Stuart A et al. (2018) Molecular mechanisms of cell death: recommendations of the Nomenclature Committee on Cell Death 2018. Cell Death Differ 25:486-541 |
| Soberanes, Saul; Misharin, Alexander V; Jairaman, Amit et al. (2018) Metformin Targets Mitochondrial Electron Transport to Reduce Air-Pollution-Induced Thrombosis. Cell Metab : |
| Wang, Chao; Balch, William E (2018) Bridging Genomics to Phenomics at Atomic Resolution through Variation Spatial Profiling. Cell Rep 24:2013-2028.e6 |
| Kong, Hyewon; Chandel, Navdeep S (2018) Regulation of redox balance in cancer and T cells. J Biol Chem 293:7499-7507 |
| Hutt, Darren M; Mishra, Sanjay Kumar; Roth, Daniela Martino et al. (2018) Silencing of the Hsp70-specific nucleotide-exchange factor BAG3 corrects the F508del-CFTR variant by restoring autophagy. J Biol Chem 293:13682-13695 |
| Hsiao, Hsi-Min; Fernandez, Ramiro; Tanaka, Satona et al. (2018) Spleen-derived classical monocytes mediate lung ischemia-reperfusion injury through IL-1?. J Clin Invest 128:2833-2847 |
| McQuattie-Pimentel, Alexandra C; Budinger, G R Scott; Ballinger, Megan N (2018) Monocyte-derived Alveolar Macrophages: The Dark Side of Lung Repair? Am J Respir Cell Mol Biol 58:5-6 |
| Hutt, Darren M; Loguercio, Salvatore; Campos, Alexandre Rosa et al. (2018) A Proteomic Variant Approach (ProVarA) for Personalized Medicine of Inherited and Somatic Disease. J Mol Biol 430:2951-2973 |
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