Abstract

The Core Laboratory will continue to provide professional and technical support for individual investigators and projects within the program to function efficiently. This support has in the past and will continue to include the following activities: 1) Harvesting and preservation of organ donor and recipient tissue, cells, serum, and other material for use in individual projects; 2) Performance and development of routine assays for component projects using serologic, ELISA, fluorochemical, cellular immune, and radioimmune techniques; 3) Support in the development of new projects and procedures, such as tissue/cell culture, cell hybridization, monoclonal antibody production, and tissue immunohistochemistry: 4) Coordination of diagnostic tests for proposed in vivo studies, including blood counts and chemistry, radiologic imaging and MRI, and tissue biopsy evaluation by histologic, ultrastructural, immunologic, and enzymatic methods.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Program Projects (P01)
Project #
5P01AI019368-08
Application #
3809876
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
8
Fiscal Year
1990
Total Cost
Indirect Cost

  Institution

Name
Duke University
Department
Type
DUNS #
071723621
City
Durham
State
NC
Country
United States
Zip Code
27705

  Publications

Kirk, A D; Bollinger, R R; Finn, O J (1995) Rapid, comprehensive analysis of human cytokine mRNA and its application to the study of acute renal allograft rejection. Hum Immunol 43:113-28
Pruitt, S K; Kirk, A D; Bollinger, R R et al. (1994) The effect of soluble complement receptor type 1 on hyperacute rejection of porcine xenografts. Transplantation 57:363-70
Pruitt, S K; Baldwin 3rd, W M; Barth, R N et al. (1993) The effect of xenoreactive antibody and B cell depletion on hyperacute rejection of guinea pig-to-rat cardiac xenografts. Transplantation 56:1318-24
Spurney, R F; Onorato, J J; Ruiz, P et al. (1993) Characterization of glomerular thromboxane receptors in murine lupus nephritis. J Pharmacol Exp Ther 264:584-90
Gravatt, L C; Chaffee, S; Hebert, M E et al. (1993) Efficacy and toxicity of 9-beta-D-arabinofuranosylguanine (araG) as an agent to purge malignant T cells from murine bone marrow: application to an in vivo T-leukemia model. Leukemia 7:1261-7
Kirk, A D; Li, R A; Kinch, M S et al. (1993) The human antiporcine cellular repertoire. In vitro studies of acquired and innate cellular responsiveness. Transplantation 55:924-31
Pruitt, S K; Weinstock, D; Suyemoto, M M et al. (1993) Effect of bursectomy on deposition of natural xenoreactive antibodies and complement within rat cardiac xenografts in the chicken. Transplant Proc 25:435-7
Messina, J P; Gilkeson, G S; Pisetsky, D S (1993) The influence of DNA structure on the in vitro stimulation of murine lymphocytes by natural and synthetic polynucleotide antigens. Cell Immunol 147:148-57
Kirk, A D; Heinle, J S; Mault, J R et al. (1993) Ex vivo characterization of human anti-porcine hyperacute cardiac rejection. Transplantation 56:785-93
Brauer, R B; Baldwin 3rd, W M; Daha, M R et al. (1993) Use of C6-deficient rats to evaluate the mechanism of hyperacute rejection of discordant cardiac xenografts. J Immunol 151:7240-8

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