Abstract

We have made the observation that peripheral T cells expressing V alpha7 genes are highly underrepresented in C57BL/10 mice. This is surprising considering V alpha7 genes comprise 8%-10% of V alpha germline gene pool. The experiments proposed in this grant application seek to determine the mechanism or process which regulates the number of V alpha7-expressing T cells in the periphery. A set os experiments is designed to determine whether the V alpha7 genes carry a structural defect that prevents their rearrangement or efficient transcription. A second set of experiments is designed to estimate the frequence of V alpha7-expressing cells among thymocytes and peripheral T cells; these experiments focus on the potential role of tolerance and/or thymic selection in determining the number of mature, peripheral V alpha7-expressing T cells.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Program Projects (P01)
Project #
5P01AI022295-08
Application #
3790999
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
8
Fiscal Year
1992
Total Cost
Indirect Cost

  Institution

Name
National Jewish Health
Department
Type
DUNS #
City
Denver
State
CO
Country
United States
Zip Code
80206

  Publications

Ruiz-Moreno, Juan Sebastian; Hamann, Lutz; Jin, Lei et al. (2018) The cGAS/STING Pathway Detects Streptococcus pneumoniae but Appears Dispensable for Antipneumococcal Defense in Mice and Humans. Infect Immun 86:
Liu, Haolin; Wang, Chao; Lee, Schuyler et al. (2018) Specific Recognition of Arginine Methylated Histone Tails by JMJD5 and JMJD7. Sci Rep 8:3275
Rubtsova, Kira; Rubtsov, Anatoly V; Thurman, Joshua M et al. (2017) B cells expressing the transcription factor T-bet drive lupus-like autoimmunity. J Clin Invest 127:1392-1404
Getahun, Andrew; Wemlinger, Scott M; Rudra, Pratyaydipta et al. (2017) Impaired B cell function during viral infections due to PTEN-mediated inhibition of the PI3K pathway. J Exp Med 214:931-941
Liu, Haolin; Wang, Chao; Lee, Schuyler et al. (2017) Clipping of arginine-methylated histone tails by JMJD5 and JMJD7. Proc Natl Acad Sci U S A 114:E7717-E7726
Lang, Julie; Ota, Takayuki; Kelly, Margot et al. (2016) Receptor editing and genetic variability in human autoreactive B cells. J Exp Med 213:93-108
Noges, Laura E; White, Janice; Cambier, John C et al. (2016) Contamination of DNase Preparations Confounds Analysis of the Role of DNA in Alum-Adjuvanted Vaccines. J Immunol 197:1221-30
Packard, Thomas A; Smith, Mia J; Conrad, Francis J et al. (2016) B Cell Receptor Affinity for Insulin Dictates Autoantigen Acquisition and B Cell Functionality in Autoimmune Diabetes. J Clin Med 5:
Morton, J Jason; Bird, Gregory; Refaeli, Yosef et al. (2016) Humanized Mouse Xenograft Models: Narrowing the Tumor-Microenvironment Gap. Cancer Res 76:6153-6158
Getahun, Andrew; Beavers, Nicole A; Larson, Sandy R et al. (2016) Continuous inhibitory signaling by both SHP-1 and SHIP-1 pathways is required to maintain unresponsiveness of anergic B cells. J Exp Med 213:751-69

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