Abstract

Flow cytometry/cell sorting is a critical resource for contemporary research in immunology and cell biology.All projects within this program, and Core 3, have extensive needs for this technology including, in somecases, needs for the same application and reagents. In view of this, we have integrated these needs in CoreA to accomplish economy of scale. Core A will provide for flow cytometry and cell sorting needs of programparticipants. This will include high rate cell sorting based on up to seven parameters (light scatter andfluorescence) and rare event analysis. The facility will also provide up to nine parameter analysis of cellsurface marker expression (immunofluorescence) and various static cell physiologic parameters (apoptosis,cell viability, and cell cycle), as well as real-time cell physiologic changes (calcium mobilization, intracellulartranslocation and mitochondrial membrane potential, etc.) with gating on additional parameters. Services willbe provided using the seven facility instruments that currently constitute the National Jewish Medical andResearch Center institutional flow cytometry facility.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Program Projects (P01)
Project #
5P01AI022295-22
Application #
7663283
Study Section
Special Emphasis Panel (ZAI1)
Project Start
2008-08-01
Project End
2012-07-31
Budget Start
2008-08-01
Budget End
2009-07-31
Support Year
22
Fiscal Year
2008
Total Cost
$119,303
Indirect Cost

  Institution

Name
National Jewish Health
Department
Type
DUNS #
076443019
City
Denver
State
CO
Country
United States
Zip Code
80206

  Publications

Ruiz-Moreno, Juan Sebastian; Hamann, Lutz; Jin, Lei et al. (2018) The cGAS/STING Pathway Detects Streptococcus pneumoniae but Appears Dispensable for Antipneumococcal Defense in Mice and Humans. Infect Immun 86:
Liu, Haolin; Wang, Chao; Lee, Schuyler et al. (2018) Specific Recognition of Arginine Methylated Histone Tails by JMJD5 and JMJD7. Sci Rep 8:3275
Rubtsova, Kira; Rubtsov, Anatoly V; Thurman, Joshua M et al. (2017) B cells expressing the transcription factor T-bet drive lupus-like autoimmunity. J Clin Invest 127:1392-1404
Getahun, Andrew; Wemlinger, Scott M; Rudra, Pratyaydipta et al. (2017) Impaired B cell function during viral infections due to PTEN-mediated inhibition of the PI3K pathway. J Exp Med 214:931-941
Liu, Haolin; Wang, Chao; Lee, Schuyler et al. (2017) Clipping of arginine-methylated histone tails by JMJD5 and JMJD7. Proc Natl Acad Sci U S A 114:E7717-E7726
Lang, Julie; Ota, Takayuki; Kelly, Margot et al. (2016) Receptor editing and genetic variability in human autoreactive B cells. J Exp Med 213:93-108
Noges, Laura E; White, Janice; Cambier, John C et al. (2016) Contamination of DNase Preparations Confounds Analysis of the Role of DNA in Alum-Adjuvanted Vaccines. J Immunol 197:1221-30
Packard, Thomas A; Smith, Mia J; Conrad, Francis J et al. (2016) B Cell Receptor Affinity for Insulin Dictates Autoantigen Acquisition and B Cell Functionality in Autoimmune Diabetes. J Clin Med 5:
Morton, J Jason; Bird, Gregory; Refaeli, Yosef et al. (2016) Humanized Mouse Xenograft Models: Narrowing the Tumor-Microenvironment Gap. Cancer Res 76:6153-6158
Getahun, Andrew; Beavers, Nicole A; Larson, Sandy R et al. (2016) Continuous inhibitory signaling by both SHP-1 and SHIP-1 pathways is required to maintain unresponsiveness of anergic B cells. J Exp Med 213:751-69

Showing the most recent 10 out of 297 publications