Abstract

The Antibody Core will provide the five program projects with polyclonal and monoclonal antibodies as requested. The availability of a centralized Core for production of these reagents will be both time and cost efficient. Specifically, the Core will generate polyclonal antisera and MAbs to surface antigens of Trichomonas vaginalis, Treponema pallidum, and host receptors for T. pallidum and Group B streptococcus. These immunological reagents will be used for the isolation, purification, and characterization of bacterial surface antigens, specific bacterial adhesins, specific host bacterial receptor domains, and epitope mapping. The Core will also generate antibodies against specific cytokines in order to assist program project #4 in delineating the role that these cytokines play in cell-mediated immune responses to Chlamydia trachomatis. Finally, for all the projects, the Antibody Core will provide an efficient means for isotypic analysis of antibodies, single-cell cloning of antigen-specific monoclonal antibodies, and large scale production of these reagents.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Program Projects (P01)
Project #
5P01AI022380-05
Application #
3814507
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
5
Fiscal Year
1989
Total Cost
Indirect Cost

  Institution

Name
University of Texas Health Science Center San Antonio
Department
Type
DUNS #
800772162
City
San Antonio
State
TX
Country
United States
Zip Code
78229

  Publications

Williams, D M; Grubbs, B G; Park-Snyder, S et al. (1996) Activation of latent transforming growth factor beta during Chlamydia trachomatis-induced murine pneumonia. Res Microbiol 147:251-62
Magee, D M; Williams, D M; Smith, J G et al. (1995) Role of CD8 T cells in primary Chlamydia infection. Infect Immun 63:516-21
Williams, D M; Grubbs, B G; Schachter, J et al. (1993) Gamma interferon levels during Chlamydia trachomatis pneumonia in mice. Infect Immun 61:3556-8
Lohman, K L; Kieber-Emmons, T; Kennedy, R C (1993) Molecular characterization and structural modeling of immunoglobulin variable regions from murine monoclonal antibodies specific for hepatitis B virus surface antigen. Mol Immunol 30:1295-306
Cox, F; Taylor, L; Eskew, E K et al. (1993) Prevention of group B streptococcal colonization and bacteremia in neonatal mice with topical vaginal inhibitors. J Infect Dis 167:1118-22
Magee, D M; Igietseme, J U; Smith, J G et al. (1993) Chlamydia trachomatis pneumonia in the severe combined immunodeficiency (SCID) mouse. Reg Immunol 5:305-11
Anderson, S A; Ostberg, L G; Ehrlich, P H et al. (1992) Characterization of private and cross-reactive idiotypes associated with human antibodies to hepatitis B surface antigen. Int Immunol 4:135-45
Magee, D M; Smith, J G; Bleicker, C A et al. (1992) Chlamydia trachomatis pneumonia induces in vivo production of interleukin-1 and -6. Infect Immun 60:1217-20
Alderete, J F; Newton, E; Dennis, C et al. (1991) The vagina of women infected with Trichomonas vaginalis has numerous proteinases and antibody to trichomonad proteinases. Genitourin Med 67:469-74
Alderete, J F; Newton, E; Dennis, C et al. (1991) Antibody in sera of patients infected with Trichomonas vaginalis is to trichomonad proteinases. Genitourin Med 67:331-4

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