Abstract

We propose to use molecular virological, immunological and genetic techniques to develop and study new vaccines and antiviral approaches. Individual projects involve: (1) the development of chimeric and bifunctional immunoglobulins to enhance and stimulate the host's immunity against influenza virus and HIV infected cells; (2) the exploration of the antisense RNA concept and the principle of ribozyme inhibition in tissue culture cells and in transgenic mice using influenza virus and retroviruses as models; (3) the development of influenza virus vaccines and vaccine vectors based on the ability to use recombinant cDNA techniques to engineer influenza viruses; and (4) the study of antisense oligonucleotides.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Program Projects (P01)
Project #
5P01AI024460-07
Application #
2062595
Study Section
Microbiology and Infectious Diseases B Subcommittee (MID)
Project Start
1988-05-01
Project End
1996-04-30
Budget Start
1994-05-01
Budget End
1996-04-30
Support Year
7
Fiscal Year
1994
Total Cost
Indirect Cost

  Institution

Name
Mount Sinai School of Medicine
Department
Microbiology/Immun/Virology
Type
Schools of Medicine
DUNS #
City
New York
State
NY
Country
United States
Zip Code
10029

  Publications

Isobe, H; Moran, T; Li, S et al. (1995) Presentation by a major histocompatibility complex class I molecule of nucleoprotein peptide expressed in two different genes of an influenza virus transfectant. J Exp Med 181:203-13
Zaghouani, H; Anderson, S A; Sperber, K E et al. (1995) Induction of antibodies to the human immunodeficiency virus type 1 by immunization of baboons with immunoglobulin molecules carrying the principal neutralizing determinant of the envelope protein. Proc Natl Acad Sci U S A 92:631-5
Rodrigues, M; Li, S; Murata, K et al. (1994) Influenza and vaccinia viruses expressing malaria CD8+ T and B cell epitopes. Comparison of their immunogenicity and capacity to induce protective immunity. J Immunol 153:4636-48
Zaghouani, H; Steinman, R; Nonacs, R et al. (1993) Presentation of a viral T cell epitope expressed in the CDR3 region of a self immunoglobulin molecule. Science 259:224-7
Macri, P; Gordon, J W (1993) Transgenic animals as tools for investigating hepatocyte gene regulation and liver disease. Prog Liver Dis 11:1-25
Kuzu, H; Kuzu, Y; Zaghouani, H et al. (1993) In vivo priming effect during various stages of ontogeny of an influenza A virus nucleoprotein peptide. Eur J Immunol 23:1397-400
Kuzu, Y; Kuzu, H; Zaghouani, H et al. (1993) Priming of cytotoxic T lymphocytes at various stages of ontogeny with transfectoma cells expressing a chimeric Ig heavy chain gene bearing an influenza virus nucleoprotein peptide. Int Immunol 5:1301-7
Brumeanu, T D; Kohanski, R; Bona, C A et al. (1993) A sensitive method to detect defined peptide among those eluted from murine MHC class II molecules. J Immunol Methods 160:65-71
Li, S; Polonis, V; Isobe, H et al. (1993) Chimeric influenza virus induces neutralizing antibodies and cytotoxic T cells against human immunodeficiency virus type 1. J Virol 67:6659-66
Li, S; Rodrigues, M; Rodriguez, D et al. (1993) Priming with recombinant influenza virus followed by administration of recombinant vaccinia virus induces CD8+ T-cell-mediated protective immunity against malaria. Proc Natl Acad Sci U S A 90:5214-8

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