A core facility is operative in this laboratory for the production and maintenance monoclonal antibodies. The core is situated in a separate room equipped with laminar flow hoods, incubators, centrifuge, refrigerators, ELISA reader, and scintillation counters. The core provides the following services that are used by all program participants: 1) Assistance with the development of new monoclonal antibodies. 2) Maintenance of a large stock of cell lines and hybridomas. 3) Production of monoclonals as titred ascites and culture supernatants, purified immunoglobulin fractions, and modified Ig's (Fab fragments and biotin or fluorescein-Ig's). 4) Production of polyclonal (rabbit) antisera. 5) Mycoplasma screens and recloning of cell lines. 6) Assistance with ELISA assays and radioimmune assays.

Project Start
Project End
Budget Start
Budget End
Support Year
2
Fiscal Year
1988
Total Cost
Indirect Cost
Name
Rockefeller University
Department
Type
DUNS #
071037113
City
New York
State
NY
Country
United States
Zip Code
10065
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